| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Turner, Roshi J (12)
|
MISAC |
29.11 |
27.82 |
-1.29 |
| 2 |
Ball, Wyatt A (12)
|
OHFAST |
30.97 |
30.46 |
-0.51 |
| 3 |
Amendol, Matthew U (12)
|
LEPS |
30.67 |
31.22 |
0.55 |
| 4 |
Gottschalk, Maxwell R (12)
|
OHFMST |
32.81 |
32.48 |
-0.33 |
| 5 |
Mcbride, Riley M (12)
|
LEUN |
32.41 |
32.63 |
0.22 |
| 5 |
Sanchez, Brady M (12)
|
OHSYL |
32.79 |
32.63 |
-0.16 |
| 7 |
Sorg, Austin S (11)
|
OHRSC |
33.07 |
34.13 |
1.06 |
| 8 |
Quisno, Benjamin E (12)
|
OHGTAC |
33.32 |
34.29 |
0.97 |
| 9 |
Al-Akhras, Yusuf K (12)
|
OHGTAC |
|
34.47 |
- |
| 10 |
Li, Max X (12)
|
MISAC |
35.84 |
34.80 |
-1.04 |
| 11 |
Peters, Cole E (11)
|
OHGTAC |
36.70 |
35.41 |
-1.29 |
| 12 |
Crooks, Maxwell M (12)
|
OHSYL |
33.22 |
35.62 |
2.40 |
| 13 |
Pelz, Cody W (11)
|
OHRSC |
36.05 |
36.23 |
0.18 |
| 14 |
Harman, Landon C (12)
|
OHSYL |
35.54 |
36.48 |
0.94 |
| 15 |
Lathrop, Thomas G (11)
|
OHGTAC |
37.67 |
37.58 |
-0.09 |
| 16 |
Poland, Nathaniel P (11)
|
OHFMST |
39.01 |
38.70 |
-0.31 |
| 17 |
Muler, Fred J (11)
|
OHSYL |
40.15 |
39.57 |
-0.58 |
| 18 |
Sattig, Fynn E (11)
|
OHFMST |
37.31 |
39.84 |
2.53 |
| 19 |
Sturdevant, Zane L (11)
|
OHGTAC |
35.87 |
39.99 |
4.12 |
| 20 |
Black, Duncan P (11)
|
OHFAST |
37.24 |
40.44 |
3.20 |
| 21 |
Sanchez, Ian J (11)
|
OHSYL |
41.90 |
41.63 |
-0.27 |
| 22 |
Hernandez, Joel * (12)
|
OHBGSC |
41.23 |
42.59 |
1.36 |
| 23 |
Young, Brady G (12)
|
OHSYL |
43.27 |
43.10 |
-0.17 |
| 24 |
Pena, Tristan J (11)
|
OHWAUS |
44.96 |
43.65 |
-1.31 |
| 25 |
O'Neill, Brennan J (12)
|
OHRSC |
44.33 |
43.72 |
-0.61 |
| 26 |
Tian, Haochen (11)
|
MISAC |
45.70 |
44.61 |
-1.09 |
| 27 |
Theophelis, George N (11)
|
MISAC |
51.65 |
45.92 |
-5.73 |
| 28 |
Rozek, Ethan M (11)
|
OHSYL |
44.26 |
47.80 |
3.54 |
| 29 |
Arter, Kenneth W (11)
|
OHFAST |
45.23 |
48.30 |
3.07 |
| 30 |
Kruszka, Michael F (11)
|
OHSOS |
58.17 |
53.19 |
-4.98 |
| 31 |
Van Vorhis, Kaleb R (11)
|
OHBGSC |
51.07 |
57.35 |
6.28 |
|
Allred, Jasper D (11)
|
OHGTAC |
|
DQ |
- |