| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Merriman, Seth E (14)
|
OHFMST |
2:22.28 |
2:33.08 |
10.80 |
| 2 |
Semancik, Jonah D (13)
|
OHGTAC |
2:32.55 |
2:34.12 |
1.57 |
| 3 |
Ankney, Xander (13)
|
OHWAUS |
|
2:35.25 |
- |
| 4 |
He, Aaron W (13)
|
OHBGSC |
2:40.76 |
2:35.32 |
-5.44 |
| 5 |
Morgan, Grayson A (14)
|
OHSOS |
2:42.31 |
2:39.54 |
-2.77 |
| 6 |
Fisher, Ewan C (14)
|
OHBGSC |
2:44.20 |
2:42.29 |
-1.91 |
| 7 |
Lazenby, Oliver H (13)
|
OHGTAC |
2:47.90 |
2:46.09 |
-1.81 |
| 8 |
Weimer, Jacob R (14)
|
OHSYL |
|
2:47.38 |
- |
| 9 |
Freeworth, Keegan C (13)
|
OHSYL |
3:02.94 |
2:49.08 |
-13.86 |
| 10 |
Rippke, Andrew C (13)
|
OHGTAC |
2:50.01 |
2:49.74 |
-0.27 |
| 11 |
Carrubba, Carlo (13)
|
OHGTAC |
2:48.66 |
2:51.38 |
2.72 |
| 12 |
Nahrwold, Nicholas L (13)
|
OHNAC |
|
2:53.60 |
- |
| 13 |
Packo, Anthony (14)
|
OHGTAC |
3:06.04 |
2:54.47 |
-11.57 |
| 14 |
Danforth, Joshua W (13)
|
OHFMST |
2:48.97 |
2:54.88 |
5.91 |
| 15 |
Kado, Takayuki (13)
|
OHFAST |
|
2:54.92 |
- |
| 16 |
Sanchez, Brady M (13)
|
OHSYL |
|
2:56.44 |
- |
| 17 |
Cody, Kyle P (14)
|
OHSYL |
3:02.52 |
2:56.65 |
-5.87 |
| 18 |
Altman, Carson J (13)
|
OHSYL |
3:01.58 |
3:01.60 |
0.02 |
| 19 |
Perrault, Harrison P (14)
|
OHFRCE |
3:09.34 |
3:02.65 |
-6.69 |
| 20 |
Feik, Kevin P (14)
|
LECCST |
3:28.85 |
3:07.21 |
-21.64 |
| 21 |
Boyer, William C (14)
|
OHGTAC |
|
3:07.86 |
- |
| 22 |
Nixon, Riley S (14)
|
OHGTAC |
|
3:11.11 |
- |
| 23 |
O'Brien, Aidan J (13)
|
OHBGSC |
|
3:11.40 |
- |
| 24 |
Sturdevant, Beau D (13)
|
OHGTAC |
3:21.29 |
3:24.10 |
2.81 |
| 25 |
Hildebrand, Aiden J (13)
|
OHBGSC |
|
3:26.74 |
- |
| 26 |
Grubbs, Caleb T (13)
|
OHBGSC |
3:28.39 |
3:28.30 |
-0.09 |
| 27 |
O'Connell, Dillon P (14)
|
OHBGSC |
|
3:35.84 |
- |
| 28 |
Storrer, Isaac J (14)
|
OHGTAC |
3:56.79 |
3:54.20 |
-2.59 |
| 29 |
Hernandez, Joel (13)
|
OHBGSC |
|
4:02.01 |
- |