| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schultz, Lindy N (14)
|
OHGTAC |
6:08.61 |
5:36.02 |
-32.59 |
| 2 |
Krueger, Faith M (13)
|
OHGTAC |
5:55.80 |
5:45.62 |
-10.18 |
| 3 |
Montgomery, Emma A (13)
|
LEUN |
5:44.67 |
5:47.38 |
2.71 |
| 4 |
Sanders, Natalie A (13)
|
OHFMST |
5:50.78 |
5:50.47 |
-0.31 |
| 5 |
Freeman, Sylvia R (13)
|
OHFMST |
5:46.03 |
5:50.56 |
4.53 |
| 6 |
Baumgartner, Lily M (13)
|
OHLYSC |
6:12.80 |
5:51.93 |
-20.87 |
| 7 |
Freeworth, Kendall L (13)
|
OHSYL |
6:03.68 |
5:52.50 |
-11.18 |
| 8 |
Fiant, Natalie G (13)
|
OHDORC |
6:05.23 |
5:53.46 |
-11.77 |
| 9 |
Atherine, Eliza (13)
|
OHFMST |
5:46.49 |
5:53.54 |
7.05 |
| 10 |
Weissling, Ava N (13)
|
OHBGSC |
6:02.44 |
6:00.37 |
-2.07 |
| 11 |
Leatherman, Abigail O (13)
|
OHBGSC |
5:59.79 |
6:01.92 |
2.13 |
| 12 |
Diller, Aubrey L (13)
|
OHFMST |
6:06.80 |
6:04.16 |
-2.64 |
| 13 |
Walsh, Brynn A (13)
|
OHSYL |
6:02.75 |
6:05.84 |
3.09 |
| 14 |
Merriman, Maggie L (14)
|
OHFMST |
5:51.68 |
6:06.56 |
14.88 |
| 15 |
Desmond, Madeline G (14)
|
OHGTAC |
5:57.01 |
6:10.00 |
12.99 |
| 16 |
Layne, Margaret S (14)
|
OHBGSC |
6:10.97 |
6:23.05 |
12.08 |
| 17 |
Baccus, Kaelah S (13)
|
OHGTAC |
5:50.52 |
6:28.87 |
38.35 |
| 18 |
Billiot, Ella K (13)
|
OHBGSC |
6:14.04 |
6:29.46 |
15.42 |
| 19 |
Hudson, Taylor M (13)
|
OHFAST |
6:14.64 |
6:32.90 |
18.26 |