| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Laycock, Charlotte I (16)
|
OHUSA |
1:05.84 |
1:06.68 |
0.84 |
| 2 |
Grespin, Margaret S (16)
|
OHGTAC |
1:08.58 |
1:08.70 |
0.12 |
| 3 |
Desmond, Madeline G (15)
|
OHGTAC |
1:13.08 |
1:09.53 |
-3.55 |
| 4 |
Adams, Gabrielle O (15)
|
OHDCST |
1:09.23 |
1:10.59 |
1.36 |
| 5 |
Boone-McDougall, Sydney E (15)
|
OHDCST |
1:10.04 |
1:11.02 |
0.98 |
| 6 |
Arledge, Lauren T (15)
|
OHEP |
1:11.96 |
1:11.49 |
-0.47 |
| 7 |
Delma, Paige O (16)
|
OHWOR |
1:08.68 |
1:11.81 |
3.13 |
| 8 |
Stith, Annaliess D (15)
|
OHGCST |
1:14.51 |
1:11.99 |
-2.52 |
| 9 |
Conrad, Brynn J (16)
|
OHUSA |
1:11.66 |
1:12.26 |
0.60 |
| 10 |
Spangler, Bethany D (15)
|
OHEP |
1:01.75 |
1:12.45 |
10.70 |
| 11 |
Kinzeler, Lauren A (16)
|
OHKCST |
1:13.87 |
1:13.72 |
-0.15 |
| 12 |
Randall, Melaina B (15)
|
OHBKYS |
1:17.27 |
1:17.20 |
-0.07 |
| 13 |
Bell, Ava E (16)
|
OHGTAC |
1:18.70 |
1:17.25 |
-1.45 |
| 14 |
Schiele, Emily N (15)
|
OHEP |
1:02.53 |
1:17.72 |
15.19 |
| 15 |
Smith, Hannah R (15)
|
OHGTAC |
1:18.24 |
1:18.39 |
0.15 |
| 16 |
Musteric, Mary E (15)
|
OHBGSC |
1:16.21 |
1:19.73 |
3.52 |
| 17 |
Tennant, Mia E (15)
|
OHSYL |
1:22.91 |
1:22.86 |
-0.05 |
| 18 |
Cunningham, Makayla L (15)
|
OHWOR |
1:18.60 |
1:31.70 |
13.10 |
|
Campbell, Nanami (16)
|
OHWOR |
1:09.18 |
NS |
- |