| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sun, Eric (10)
|
OHFMST |
48.48 |
47.90 |
-0.58 |
| 2 |
Liu, Ethan V (10)
|
OHDCST |
48.65 |
48.71 |
0.06 |
| 3 |
Naprawa, Harper B (10)
|
OHGTAC |
1:01.29 |
50.63 |
-10.66 |
| 4 |
Sorg, Avery S (8)
|
OHRSC |
50.04 |
50.95 |
0.91 |
| 5 |
Asao-Kim, Luke J (9)
|
OHDCST |
1:03.33 |
51.98 |
-11.35 |
| 6 |
Powers, Liam J (9)
|
OHUSA |
51.56 |
52.35 |
0.79 |
| 7 |
Moore, Matthew S (9)
|
OHUSA |
53.50 |
52.37 |
-1.13 |
| 8 |
Woltman-Frollini, Connor T (10)
|
OHBKYS |
56.44 |
54.20 |
-2.24 |
| 9 |
Boone-McDougall, Kyle A (10)
|
OHDCST |
55.71 |
54.23 |
-1.48 |
| 10 |
Ferri, Luca U (10)
|
OHKCST |
52.00 |
58.15 |
6.15 |
| 11 |
Oswiecinski, Ryan M (8)
|
OHDCST |
1:03.50 |
59.90 |
-3.60 |
| 12 |
Liu, Aaron (8)
|
OHSYL |
1:15.45 |
1:22.13 |
6.68 |
|
Kammeyer, William T (6)
|
OHGTAC |
1:45.00 |
DQ |
- |