| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burkey, Paige G (12)
|
OHFMST |
2:23.82 |
2:23.67 |
-0.15 |
| 2 |
Bowman, Emma O (12)
|
OHUSA |
2:28.07 |
2:24.16 |
-3.91 |
| 3 |
Hyslop, Lillian J (12)
|
OHGCST |
2:26.49 |
2:24.70 |
-1.79 |
| 4 |
Daugherty, Addison I (12)
|
OHUSA |
2:45.91 |
2:25.17 |
-20.74 |
| 5 |
Powers, Kiera N (11)
|
OHUSA |
2:29.74 |
2:27.93 |
-1.81 |
| 7 |
Brown, Jessica A (12)
|
OHDCST |
2:28.33 |
2:35.41 |
7.08 |
| 8 |
Nederveld, Anneke P (11)
|
OHBKYS |
2:40.03 |
2:40.01 |
-0.02 |
| 9 |
Benz, Elena R (12)
|
OHBKYS |
2:45.88 |
2:41.81 |
-4.07 |
| 10 |
Martin, Caroline A (12)
|
OHDCST |
3:06.62 |
2:43.96 |
-22.66 |
| 11 |
Claggett, Brookelyn O (12)
|
OHBKYS |
2:42.64 |
2:46.02 |
3.38 |
| 12 |
Seelandt, Ella M (12)
|
OHBKYS |
2:51.58 |
2:46.82 |
-4.76 |
| 13 |
Pyles, Kennedy P (12)
|
OHUSA |
2:56.37 |
2:49.76 |
-6.61 |
| 14 |
Gottschalk, Macy R (11)
|
OHFMST |
2:52.14 |
2:52.73 |
0.59 |
| 15 |
Brown, Gabriella F (12)
|
OHUSA |
3:01.37 |
2:55.12 |
-6.25 |
| 16 |
Otchy, Veronica G (12)
|
OHKCST |
3:13.08 |
3:00.32 |
-12.76 |
| 17 |
Vaughan, Alexandra L (11)
|
OHGCST |
2:59.99 |
3:02.46 |
2.47 |
| 18 |
Drerup, Claire E (12)
|
OHGTAC |
3:20.09 |
3:33.37 |
13.28 |