| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trumpower, Adayaleise L (7)
|
OHUN |
43.69 |
42.15 |
-1.54 |
| 2 |
Dyussemaliyeva, Laila R (8)
|
OHWOR |
43.22 |
42.74 |
-0.48 |
| 3 |
Bigelow, Emmerson G (8)
|
OHFMST |
48.90 |
45.80 |
-3.10 |
| 4 |
Castrillon-Gomez, Sofia (7)
|
OHFMST |
50.00 |
46.49 |
-3.51 |
| 5 |
Kessler, Kieradyn M (8)
|
OHFMST |
48.85 |
47.61 |
-1.24 |
| 6 |
Stannert, Lillian G (8)
|
OHFMST |
50.00 |
48.25 |
-1.75 |
| 7 |
Sunday, Alaina J (7)
|
OHFMST |
|
48.95 |
- |
| 8 |
Lopez, Jennifer * (8)
|
OHBGSC |
51.65 |
51.51 |
-0.14 |
| 9 |
Garon, Hailey R (7)
|
OHBGSC |
|
53.47 |
- |
| 10 |
Johnson, Jaylee P (7)
|
OHNAC |
|
54.42 |
- |
| 11 |
Lim, Sharon Y (7)
|
OHSYL |
|
54.88 |
- |
| 12 |
Rando, Maylie P (8)
|
OHSOS |
59.83 |
55.53 |
-4.30 |
| 13 |
Shawver, Lillian K (8)
|
OHBGSC |
55.47 |
58.97 |
3.50 |
| 14 |
Misra, Kaliani R (6)
|
OHGTAC |
1:16.14 |
1:13.94 |
-2.20 |
|
Alt, Eden E (8)
|
OHBGSC |
|
DQ |
- |
|
Misra, Karmina R (5)
|
OHGTAC |
2:33.23 |
DQ |
- |
|
Rhoades, Emilia M (8)
|
OHSOS |
56.85 |
DQ |
- |