| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Greinke, Elijah R (14)
|
KYBYB |
30.36 |
28.13 |
-2.23 |
| 2 |
McMasters, William M (14)
|
OHLYST |
29.04 |
29.17 |
0.13 |
| 3 |
Schweikert, Andrew W (14)
|
LEGYB |
28.86 |
29.39 |
0.53 |
| 4 |
MacKillop, Patrick S (13)
|
MIBBD |
31.09 |
29.45 |
-1.64 |
| 5 |
Wiater, Joseph D (14)
|
MIBBD |
31.77 |
29.94 |
-1.83 |
| 6 |
Nickoli, Trey (14)
|
AYAA |
35.96 |
30.03 |
-5.93 |
| 7 |
Anger, Isaac D (13)
|
WVRAYS |
30.96 |
30.06 |
-0.90 |
| 8 |
Honigford, Ross W (13)
|
NMYWSC |
30.38 |
30.37 |
-0.01 |
| 9 |
Luehmann, Niklas V (13)
|
MIRAYS |
31.50 |
30.92 |
-0.58 |
| 10 |
Danforth, Joshua W (14)
|
OHFMST |
33.76 |
32.21 |
-1.55 |
| 11 |
Kilpatrick, Brayden M (13)
|
MILCY |
32.49 |
32.46 |
-0.03 |
| 12 |
Kowalski, Cooper J (14)
|
INSTRS |
33.12 |
32.56 |
-0.56 |
| 13 |
Sutter, Landon (13)
|
OHGCYD |
37.82 |
32.93 |
-4.89 |
| 14 |
D'Errico, Dominic M (14)
|
OHLYST |
33.13 |
33.21 |
0.08 |
| 15 |
Koh, Thomas D (13)
|
OHLYSC |
32.75 |
33.41 |
0.66 |
| 16 |
Diller, Xavier D (13)
|
OHLYSC |
34.00 |
35.07 |
1.07 |