| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kuchrakova, Yulduz (27)
|
OHCATS |
26.58 |
27.09 |
0.51 |
| 2 |
Oyster, Olivia G (17)
|
OHUSA |
27.23 |
27.54 |
0.31 |
| 3 |
DeCarlo, Evelyn E (17)
|
OHWOR |
28.28 |
28.41 |
0.13 |
| 4 |
Desmond, Michaela A (17)
|
OHGTAC |
28.75 |
29.16 |
0.41 |
| 5 |
Saxe, Laney A (17)
|
OHSYL |
29.90 |
29.17 |
-0.73 |
| 6 |
Butnariu, Alexandra (17)
|
OHGCST |
28.95 |
29.36 |
0.41 |
| 7 |
Richner, Lauren E (17)
|
OHWOR |
28.27 |
29.42 |
1.15 |
| 7 |
Lichtner, Kasey E (18)
|
OHGCST |
28.45 |
29.42 |
0.97 |
| 9 |
Smith, Lauren A (17)
|
OHUSA |
30.20 |
29.81 |
-0.39 |
| 10 |
Loughrey, Kirin K (17)
|
OHUSA |
29.29 |
29.83 |
0.54 |
| 11 |
Rousher, Callista M (17)
|
OHWOR |
29.44 |
29.89 |
0.45 |
| 12 |
Wells, Amelia M (17)
|
OHLTPY |
31.65 |
30.27 |
-1.38 |
| 13 |
Spangler, Emma H (17)
|
OHEP |
27.87 |
30.70 |
2.83 |
| 14 |
Chytil, Lauren K (17)
|
OHSYL |
30.31 |
31.19 |
0.88 |
| 15 |
Dauer, Ahnika L (17)
|
OHUSA |
31.64 |
31.29 |
-0.35 |
| 16 |
Simashkevich, Kayla L (17)
|
OHBKYS |
31.07 |
31.62 |
0.55 |
| 17 |
Rivera, Kendal M (17)
|
OHGTAC |
32.20 |
32.25 |
0.05 |
| 18 |
Turner, Kailee A (17)
|
OHGTAC |
32.19 |
32.29 |
0.10 |
| 19 |
Storrer, Gabrielle M (17)
|
OHGTAC |
34.04 |
33.75 |
-0.29 |
| 20 |
Heym, Caroline N (17)
|
OHLTPY |
32.87 |
36.69 |
3.82 |