| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Klein, Tyler A (14)
|
OHBKYS |
2:48.48 |
2:42.81 |
-5.67 |
| 2 |
Van Cott, Joshua T (16)
|
OHSYL |
2:33.79 |
2:45.28 |
11.49 |
| 3 |
Tseng, Chih-Hsiun A (14)
|
OHUNOH |
2:44.34 |
2:47.06 |
2.72 |
| 4 |
Rutledge-Jukes, Keegan I (17)
|
OHSYL |
2:35.55 |
2:48.01 |
12.46 |
| 5 |
Peck, Emily M (19)
|
KYHA |
2:40.21 |
2:52.99 |
12.78 |
| 6 |
Livesay, Elizabeth E (17)
|
KYHA |
2:47.08 |
2:56.96 |
9.88 |
| 7 |
Liu, David (14)
|
OHGTAC |
3:11.36 |
3:01.49 |
-9.87 |
| 8 |
Jones, Ethan J (16)
|
OHBGSC |
2:47.47 |
3:01.91 |
14.44 |
| 9 |
Ok, Aiden J (14)
|
OHLYSC |
3:01.64 |
3:01.99 |
0.35 |
| 10 |
Mowery, Brayden C (13)
|
OHGTAC |
2:50.60 |
3:02.24 |
11.64 |
| 11 |
Dietrich, Tobias J (14)
|
OHUNOH |
3:08.24 |
3:03.62 |
-4.62 |
| 12 |
Rippke, Andrew C (15)
|
OHGTAC |
2:55.74 |
3:04.92 |
9.18 |
| 13 |
Sullivan, Alex E (14)
|
OHBKYS |
3:15.00 |
3:05.46 |
-9.54 |
| 14 |
McCubbin, Macie G (16)
|
KYHA |
3:03.73 |
3:05.70 |
1.97 |
| 15 |
Van Frayen, Brody M (14)
|
OHBKYS |
3:04.76 |
3:05.76 |
1.00 |
| 15 |
Zipes, Laura J (17)
|
OHWTRC |
2:57.37 |
3:05.76 |
8.39 |
| 17 |
Paskiet, Sophia E (15)
|
OHGTAC |
3:21.38 |
3:09.71 |
-11.67 |
| 18 |
Smith, Kailyn (17)
|
OHBKYS |
3:10.00 |
3:10.04 |
0.04 |
| 19 |
Long, Annabel E (14)
|
OHLTPY |
3:12.26 |
3:12.03 |
-0.23 |
| 20 |
Patterson, Christopher C (17)
|
OHLTPY |
3:06.10 |
3:13.27 |
7.17 |
| 21 |
Carle, Wyeth B (13)
|
OHBKYS |
3:05.65 |
3:13.28 |
7.63 |
| 22 |
O'Brien, Aiden J (14)
|
OHBGSC |
3:14.80 |
3:13.78 |
-1.02 |
| 23 |
Harman, Landon C (14)
|
OHGTAC |
3:15.64 |
3:15.16 |
-0.48 |
| 24 |
Kline, Kendal C (13)
|
OHBKYS |
3:28.62 |
3:15.56 |
-13.06 |
| 25 |
Towle, Ethan J (15)
|
OHKCST |
3:22.89 |
3:15.95 |
-6.94 |
| 26 |
Schmidt, Cienna G (13)
|
OHKCST |
3:17.15 |
3:16.90 |
-0.25 |
| 27 |
Chen, Alexander K (18)
|
OHLTPY |
3:13.72 |
3:17.25 |
3.53 |
| 28 |
Peters, Cole E (13)
|
OHGTAC |
3:27.05 |
3:18.16 |
-8.89 |
| 29 |
Randall, Melaina B (15)
|
OHBKYS |
3:15.28 |
3:19.17 |
3.89 |
| 30 |
Turner, Kailee A (17)
|
OHGTAC |
3:28.72 |
3:19.39 |
-9.33 |
| 31 |
Tan, Benjamin W (14)
|
OHLTPY |
3:26.46 |
3:21.52 |
-4.94 |
| 32 |
Gehring, Margaret G (17)
|
OHLTPY |
3:28.00 |
3:24.82 |
-3.18 |
| 33 |
Smith, Caleb (16)
|
OHBKYS |
3:53.30 |
3:27.96 |
-25.34 |
| 34 |
Nixon, Audrey G (13)
|
OHGTAC |
3:52.86 |
3:28.19 |
-24.67 |
| 35 |
Madsen, Natalie I (13)
|
OHLTPY |
3:31.75 |
3:35.29 |
3.54 |
| 36 |
Biesiada, Charlotte D (13)
|
OHFAST |
4:12.34 |
3:42.20 |
-30.14 |
| 37 |
Weber, Matthew B (15)
|
OHFAST |
3:50.10 |
3:47.27 |
-2.83 |
| 38 |
Otchy, Veronica G (12)
|
OHKCST |
3:50.00 |
4:52.03 |
62.03 |