| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Van Frayen, Brody M (14)
|
OHBKYS |
5:28.02 |
5:34.68 |
6.66 |
| 2 |
Zipes, Laura J (17)
|
OHWTRC |
5:24.50 |
5:37.14 |
12.64 |
| 3 |
Ok, Niel J (16)
|
OHLYSC |
5:37.79 |
5:44.33 |
6.54 |
| 4 |
Tseng, Chih-Hsiang J (18)
|
OHLTPY |
5:26.05 |
5:44.68 |
18.63 |
| 5 |
Tseng, Chih-Hsiun A (14)
|
OHUNOH |
5:48.66 |
5:44.79 |
-3.87 |
| 6 |
Long, Annabel E (14)
|
OHLTPY |
5:46.43 |
5:45.58 |
-0.85 |
| 7 |
Ok, Aiden J (14)
|
OHLYSC |
5:48.37 |
5:47.76 |
-0.61 |
| 8 |
Smith, Kailyn (17)
|
OHBKYS |
5:50.56 |
5:48.52 |
-2.04 |
| 9 |
McCubbin, Macie G (16)
|
KYHA |
5:48.22 |
5:51.71 |
3.49 |
| 10 |
Drzewiecki, Samantha K (16)
|
OHSYL |
6:11.09 |
6:14.68 |
3.59 |
| 11 |
Kline, Kendal C (13)
|
OHBKYS |
7:50.43 |
6:17.09 |
-93.34 |
| 12 |
Cirman, Carly N (14)
|
OHBKYS |
6:08.78 |
6:25.83 |
17.05 |
| 13 |
Zhu, Ryan S (14)
|
OHLTPY |
6:40.94 |
6:31.78 |
-9.16 |
| 14 |
Schmidt, Cienna G (13)
|
OHKCST |
6:30.00 |
6:32.94 |
2.94 |
| 15 |
Crandell, Cameron M (13)
|
OHGTAC |
6:12.77 |
6:34.78 |
22.01 |
| 16 |
Otchy, Georgiana J (14)
|
OHKCST |
6:40.00 |
7:22.60 |
42.60 |