| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brooks, Heidi J (16)
|
KYUN |
5:17.09 |
5:09.85 |
-7.24 |
| 2 |
Zinobile, Allison C (18)
|
KYRACE |
5:01.43 |
5:17.53 |
16.10 |
| 3 |
Witcher, Leah F (16)
|
KYRACE |
5:11.26 |
5:20.22 |
8.96 |
| 4 |
Wheeler, Carolina G (14)
|
KYRACE |
5:21.09 |
5:29.28 |
8.19 |
| 5 |
Gardner, Hannah M (15)
|
KYRACE |
5:37.89 |
5:31.84 |
-6.05 |
| 6 |
Barbee, Madeline E (14)
|
KYRACE |
|
5:35.46 |
- |
| 7 |
Noyes, Penelope P (65)
|
KYSKY |
5:20.45 |
5:36.64 |
16.19 |
| 8 |
Gregory-Mabrey, Ava M (15)
|
KYRACE |
5:33.04 |
5:40.70 |
7.66 |
| 9 |
Calloway, Caroline B (13)
|
KYRACE |
5:51.81 |
5:51.01 |
-0.80 |
| 10 |
Matzke, Amelia K (13)
|
KYRACE |
|
6:22.70 |
- |
| 11 |
Gregory-Mabrey, Quinn S (13)
|
KYRACE |
|
6:32.77 |
- |