| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bigelow, Emmerson G (8)
|
OHFMST |
18.40 |
17.91 |
-0.49 |
| 2 |
Lindhorst, Charlotte C (8)
|
OHSYL |
18.04 |
18.70 |
0.66 |
| 3 |
Lopez, Jennifer (8)
|
OHBGSC |
20.59 |
19.12 |
-1.47 |
| 4 |
Kin, Emily L (7)
|
OHFAST |
21.28 |
19.14 |
-2.14 |
| 5 |
Burkey, Brynn M (8)
|
OHFMST |
19.45 |
19.47 |
0.02 |
| 6 |
Kessler, Kieradyn M (8)
|
OHFMST |
20.00 |
19.69 |
-0.31 |
| 7 |
Cheatwood, Katelyn J (8)
|
OHGTAC |
22.40 |
19.92 |
-2.48 |
| 8 |
Fritz, Alexa J (8)
|
LEPS |
21.19 |
20.09 |
-1.10 |
| 9 |
Hutchinson, Julia C (8)
|
OHUN |
21.75 |
20.42 |
-1.33 |
| 10 |
Shawver, Lillian K (8)
|
OHBGSC |
20.85 |
20.47 |
-0.38 |
| 11 |
Majerski, Amelia G (8)
|
OHGTAC |
|
22.31 |
- |
| 12 |
Rando, Maylie P (8)
|
OHSOS |
25.43 |
22.41 |
-3.02 |
| 13 |
Eisenhauer, Brooklynn G (7)
|
OHSOS |
|
28.46 |
- |
| 14 |
Misra, Kaliani R (6)
|
OHGTAC |
41.91 |
30.15 |
-11.76 |
| 15 |
Hernandez, Melody * (7)
|
OHBGSC |
30.70 |
31.36 |
0.66 |
| 16 |
Misra, Karmina R (4)
|
OHGTAC |
|
47.01 |
- |
|
Trumpower, Adayaleise L (7)
|
OHUN |
16.12 |
15.10 |
-1.02 |
|
Rhoades, Emilia M (8)
|
OHSOS |
21.91 |
NS |
- |