| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trumpower, Adayaleise L (7)
|
OHUN |
47.59 |
44.16 |
-3.43 |
| 2 |
Kessler, Kieradyn M (8)
|
OHFMST |
|
48.85 |
- |
| 3 |
Bigelow, Emmerson G (8)
|
OHFMST |
|
48.90 |
- |
| 4 |
Burkey, Brynn M (8)
|
OHFMST |
|
49.25 |
- |
| 5 |
Kin, Emily L (7)
|
OHFAST |
|
49.85 |
- |
| 6 |
Lindhorst, Charlotte C (8)
|
OHSYL |
56.97 |
50.81 |
-6.16 |
| 7 |
Hutchinson, Julia C (8)
|
OHUN |
56.39 |
51.89 |
-4.50 |
| 8 |
Lopez, Jennifer (8)
|
OHBGSC |
|
54.66 |
- |
| 9 |
Shawver, Lillian K (8)
|
OHBGSC |
57.71 |
55.47 |
-2.24 |
| 10 |
Eisenhauer, Brooklynn G (7)
|
OHSOS |
|
57.30 |
- |
| 11 |
Cheatwood, Katelyn J (8)
|
OHGTAC |
1:01.53 |
58.56 |
-2.97 |
| 12 |
Rando, Maylie P (8)
|
OHSOS |
|
1:00.67 |
- |
| 13 |
Majerski, Amelia G (8)
|
OHGTAC |
|
1:06.96 |
- |
| 14 |
Hernandez, Melody * (7)
|
OHBGSC |
1:09.23 |
1:09.33 |
0.10 |
| 15 |
Misra, Kaliani R (6)
|
OHGTAC |
1:36.23 |
1:16.14 |
-20.09 |
| 16 |
Misra, Karmina R (4)
|
OHGTAC |
|
2:33.23 |
- |
|
Rhoades, Emilia M (8)
|
OHSOS |
58.97 |
NS |
- |