| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sangasy, Khany K (11)
|
NISEAS |
29.55 |
30.45 |
0.90 |
| 2 |
Langan, Abigayle R (11)
|
NIVICT |
32.19 |
32.47 |
0.28 |
| 3 |
Barry, Madeline G (11)
|
NIVICT |
34.00 |
34.65 |
0.65 |
| 4 |
Hager, William N (10)
|
NIGCOM |
40.05 |
35.38 |
-4.67 |
| 5 |
Wagner, Morgan E (9)
|
NIVICT |
34.23 |
35.49 |
1.26 |
| 6 |
Campbell, Brooklyn M (11)
|
NIVICT |
40.99 |
41.09 |
0.10 |
| 7 |
Crossing, Natalie E (9)
|
NIVICT |
39.83 |
41.67 |
1.84 |
| 8 |
Pogroszewski, Frances S (9)
|
NISEAS |
50.58 |
44.17 |
-6.41 |
| 9 |
Stahl, Reed P (9)
|
NIVICT |
42.20 |
44.72 |
2.52 |
| 10 |
Nie, Amy (10)
|
NISEAS |
48.42 |
45.83 |
-2.59 |
| 11 |
Noble, Mia J (9)
|
NISEAS |
|
46.13 |
- |
| 12 |
Tripp, Sophia G (10)
|
NIVICT |
48.59 |
47.41 |
-1.18 |
| 13 |
Ray, Gavin M (9)
|
NIVICT |
47.05 |
52.07 |
5.02 |
| 14 |
Cottone, Cameron A (9)
|
NIGCOM |
52.46 |
54.93 |
2.47 |
| 15 |
Holly, Gaige W (10)
|
NIGCOM |
54.15 |
57.82 |
3.67 |
| 16 |
Healy, Owen D (10)
|
NIVICT |
54.80 |
58.29 |
3.49 |