| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gillan, Sierra P (13)
|
MRDVWM |
25.17 |
25.12 |
-0.05 |
| 2 |
Muszynski, Amanda (14)
|
MRNYCC |
25.45 |
25.45 |
0.00 |
| 3 |
Adams, Grace C (13)
|
MRTRI |
25.99 |
25.50 |
-0.49 |
| 4 |
Iannetta, Ava L (14)
|
MRWEST |
25.99 |
25.71 |
-0.28 |
| 5 |
Horn, Ursula (13)
|
MRMAKO |
25.95 |
25.76 |
-0.19 |
| 6 |
Tennant, Skylar M (13)
|
MRBAD |
25.80 |
25.90 |
0.10 |
| 7 |
Walsh, Stephanie M (14)
|
MRLIE |
25.98 |
25.91 |
-0.07 |
| 8 |
Mazzacano, Emmy R (14)
|
MRPAC |
25.63 |
25.93 |
0.30 |
| 9 |
Boals, Sydney E (13)
|
MRTVSC |
25.37 |
26.06 |
0.69 |
| 10 |
Donlevy, Margaret M (13)
|
MRCIY |
25.31 |
26.08 |
0.77 |
| 11 |
Ehlers, Kaitlyn R (13)
|
MRTVSC |
26.11 |
26.37 |
0.26 |
| 12 |
Armistead, Allison H (14)
|
MRAG |
26.16 |
26.46 |
0.30 |
| 13 |
McIntyre, Gaby M (13)
|
MRLIE |
25.98 |
26.50 |
0.52 |
| 14 |
Pellegrino, Francesca (14)
|
MRTVSC |
26.37 |
26.52 |
0.15 |
| 15 |
Lopez, Olivia K (13)
|
MRPAC |
26.05 |
26.55 |
0.50 |
| 16 |
Thoms, Julia D (13)
|
MRLIE |
26.14 |
26.59 |
0.45 |
| 17 |
Rojas-Tineo, Mairene R (13)
|
MRAPEX |
26.26 |
26.62 |
0.36 |
| 18 |
Claps, Emma (14)
|
MRHAA |
26.33 |
26.74 |
0.41 |
| 19 |
Crane, Rebecca A (14)
|
MRIA |
25.86 |
26.78 |
0.92 |