| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ayupova, Maya (13)
|
MRSHY |
41.74 |
39.78 |
-1.96 |
| 2 |
Seymour, Nayelly (13)
|
NJWWAT |
40.77 |
41.06 |
0.29 |
| 3 |
Nathanson, Michelle (14)
|
MRFREE |
43.20 |
41.70 |
-1.50 |
| 4 |
Forbin, Zara A (14)
|
MRBKR |
45.81 |
42.17 |
-3.64 |
| 5 |
Colangelo-Ghossein, Naya B (14)
|
MRBKR |
45.00 |
42.82 |
-2.18 |
| 6 |
Troncoso, Emma H (13)
|
MRFREE |
45.42 |
44.15 |
-1.27 |
| 7 |
Gelashvili, Elizaveta L (13)
|
MRMPNY |
42.00 |
44.65 |
2.65 |
| 8 |
McGee, Leah T (13)
|
MRBKR |
44.90 |
45.66 |
0.76 |
| 9 |
Hall, Emma B (13)
|
MRBKR |
44.02 |
45.94 |
1.92 |
| 10 |
Albert, Victoria E (13)
|
MRBKR |
48.15 |
48.04 |
-0.11 |
|
Sanchez, Valentina S (13)
|
MRFREE |
48.66 |
DQ |
- |
|
Epps, Emani N (13)
|
MRBKR |
41.46 |
NS |
- |