| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Weingarten, Elise A (10)
|
MNRSC |
2:38.65 |
2:34.21 |
-4.44 |
| 2 |
Nymo, Sigrid (9)
|
MNEDI |
2:48.41 |
2:39.39 |
-9.02 |
| 3 |
Seder, Katelyn V (10)
|
WIASC |
2:57.49 |
2:42.25 |
-15.24 |
| 4 |
Bruett, Sarah E (10)
|
WISHOR |
2:49.72 |
2:44.36 |
-5.36 |
| 5 |
Leon, Erin H (10)
|
WIWYNS |
2:50.17 |
2:49.12 |
-1.05 |
| 6 |
Maclachlan, Gwenyth E (10)
|
MNEDI |
2:57.77 |
2:53.57 |
-4.20 |
| 7 |
DuBois, Madeline E (9)
|
WISHOR |
2:58.46 |
2:57.77 |
-0.69 |
| 8 |
Pagels, Hannah C (9)
|
WISHOR |
3:00.24 |
2:58.04 |
-2.20 |
| 9 |
Kwiatkowski, Emily M (10)
|
WIOZ |
3:05.14 |
2:59.62 |
-5.52 |
| 10 |
Dickinson, Margaret R (9)
|
WISSTY |
3:37.62 |
3:00.14 |
-37.48 |
| 11 |
Clausman, Isabella F (10)
|
MNFOXJ |
3:13.11 |
3:03.06 |
-10.05 |
| 12 |
Miota, Tess K (10)
|
WISHOR |
2:57.28 |
3:03.73 |
6.45 |
| 13 |
Hall, Grace A (9)
|
MNEDI |
3:06.52 |
3:04.66 |
-1.86 |
| 14 |
Bonney, Olivia M (9)
|
WILAKE |
4:17.93 |
3:26.06 |
-51.87 |
| 15 |
Lampi, Kalia M (9)
|
WILAKE |
4:36.87 |
3:55.70 |
-41.17 |
| 16 |
Yan, Lorraine (9)
|
WILAKE |
|
4:03.40 |
- |
|
Springer, Carly F (10)
|
WIWYNS |
|
3:03.67 |
- |