| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Paradis, Harper E (8)
|
WIGCY |
21.38 |
20.10 |
-1.28 |
| 2 |
Matuszewski, Kailey P (8)
|
DCNLY |
21.32 |
20.92 |
-0.40 |
| 3 |
Brostowitz, Elizabeth A (7)
|
WISSTY |
23.08 |
21.47 |
-1.61 |
| 4 |
Michalski, Amelia O (8)
|
WIDCY |
21.86 |
21.68 |
-0.18 |
| 5 |
Schulte, Grace (8)
|
WIMYST |
22.07 |
21.69 |
-0.38 |
| 6 |
Branville, Callie (8)
|
WICVY |
22.21 |
22.30 |
0.09 |
| 7 |
Chapman, Avery J (8)
|
WILAXY |
22.15 |
22.32 |
0.17 |
| 8 |
Shunk, Hadleigh M (8)
|
WIGCY |
22.00 |
22.41 |
0.41 |
| 9 |
Toppe, Lydia M (8)
|
WISSTY |
22.48 |
22.57 |
0.09 |
| 10 |
Knowles, Olivia (8)
|
WISCY |
23.28 |
22.83 |
-0.45 |
| 11 |
Lorenz, Anna L (8)
|
WISSTY |
22.74 |
22.90 |
0.16 |
| 12 |
Kort, Lydia (8)
|
WISPDY |
23.18 |
23.08 |
-0.10 |
| 13 |
Goes, Kennedy (8)
|
WISCY |
22.67 |
23.13 |
0.46 |
| 14 |
Swetz-Wanta, Sophia (8)
|
WISPDY |
23.24 |
23.27 |
0.03 |
| 15 |
Verhelst, Maya K (8)
|
WIYDC |
23.49 |
23.69 |
0.20 |
| 16 |
Arnett, Lauryn R (8)
|
WIGCY |
22.77 |
23.71 |
0.94 |
| 17 |
Maynard, Holly A (8)
|
WIGCY |
23.90 |
24.04 |
0.14 |
| 18 |
Ringhand, Kailyn T (7)
|
WIGCY |
23.61 |
24.23 |
0.62 |
| 19 |
Ochs, Naomi (8)
|
WISCY |
24.03 |
24.54 |
0.51 |
| 20 |
Leon, Erin H (8)
|
WIWYNS |
22.97 |
24.67 |
1.70 |
| 21 |
Goldbach, Emma R (8)
|
WIECY |
23.25 |
24.89 |
1.64 |
| 22 |
Branville, Elise (8)
|
WICVY |
24.35 |
25.42 |
1.07 |
| 23 |
Schley, Audrie L (8)
|
WIDCY |
25.75 |
25.54 |
-0.21 |
| 24 |
Sved, Lola A (8)
|
MIMQTY |
26.08 |
25.88 |
-0.20 |
| 25 |
Paull, Violet B (8)
|
WISSTY |
27.24 |
26.04 |
-1.20 |
| 26 |
Schetter, Madelyn Q (7)
|
MIMQTY |
25.75 |
27.00 |
1.25 |
|
Duchenko, Nickole A (8)
|
WISSTY |
22.88 |
NS |
- |