| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Felsman, Ashley L (16)
|
WISSTY |
42.09 |
27.79 |
-14.30 |
| 2 |
Badillo, Sofia (14)
|
WISEA |
29.15 |
28.25 |
-0.90 |
| 3 |
D'Alessandro, Zoe R (14)
|
WISEA |
30.32 |
29.67 |
-0.65 |
| 4 |
Thomas, MacKenzie N (13)
|
WISEA |
35.63 |
29.89 |
-5.74 |
| 5 |
Reischl, Mackenzie J (17)
|
WISEA |
29.45 |
30.10 |
0.65 |
| 6 |
McEvilly, Savannah N (12)
|
WISSTY |
35.31 |
35.36 |
0.05 |
| 7 |
Bergemann, Rylie B (13)
|
WISEA |
33.66 |
36.13 |
2.47 |
| 8 |
Tanner, Alyssa L (12)
|
WISSTY |
39.19 |
36.90 |
-2.29 |
| 9 |
Brazzale, Elise (9)
|
WISCY |
35.40 |
37.07 |
1.67 |
| 10 |
Prokhin, Anna M (12)
|
WISSTY |
37.05 |
37.31 |
0.26 |
| 11 |
Dutta, Keya (14)
|
WISSTY |
41.65 |
39.80 |
-1.85 |
| 12 |
Palmer, Mia J (10)
|
WIGYD |
40.26 |
40.50 |
0.24 |
| 13 |
Abukhamireh, Mona K (11)
|
WISSTY |
|
41.60 |
- |
| 14 |
Braa, Julia A (12)
|
WIGYD |
48.89 |
42.72 |
-6.17 |
| 15 |
Paull, Violet B (10)
|
WISSTY |
45.58 |
44.57 |
-1.01 |
| 16 |
Reppa, Nikita M (12)
|
WISSTY |
51.86 |
47.36 |
-4.50 |
| 17 |
Klug, Isabella G (11)
|
WISSTY |
49.68 |
47.54 |
-2.14 |
| 18 |
Liu, Iris Y (9)
|
WISSTY |
49.08 |
47.85 |
-1.23 |
| 19 |
Keefe, Margaret R (13)
|
WIGYD |
50.58 |
48.66 |
-1.92 |
| 20 |
Smith, Amara J (8)
|
WISSTY |
51.23 |
51.54 |
0.31 |
| 21 |
Marquardt, Molly E (9)
|
WIOCON |
1:36.27 |
1:30.60 |
-5.67 |
|
Buhler, Isabelle (17)
|
WISEA |
32.09 |
NS |
- |
|
Pavelchik, Shayla B (11)
|
WISSTY |
45.00 |
NS |
- |
|
Graves, Alyssa J (17)
|
WISSTY |
26.64 |
NS |
- |