| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hansen, Tanner A (12)
|
WILAKE |
33.06 |
33.65 |
0.59 |
| 2 |
Hamann, Adyn L (12)
|
WIEBSC |
37.52 |
35.59 |
-1.93 |
| 3 |
Nikitovic, Maximus S (12)
|
WIEBSC |
36.92 |
36.33 |
-0.59 |
| 4 |
Haeuser, Mateo D (12)
|
WILAKE |
37.78 |
36.58 |
-1.20 |
| 5 |
Mason, Carter M (12)
|
WIMFSC |
37.92 |
37.78 |
-0.14 |
| 6 |
Esteves, Jackson L (12)
|
WIMAC |
37.51 |
38.03 |
0.52 |
| 7 |
Stich, Jackson D (12)
|
WIEBSC |
38.95 |
39.11 |
0.16 |
| 8 |
Schelfout, Aiden R (12)
|
WIMFSC |
39.48 |
40.53 |
1.05 |
| 9 |
Stanley, Clayton M (12)
|
WIMFSC |
41.42 |
41.06 |
-0.36 |
| 10 |
Shanks, Bennett C (12)
|
WISHOR |
40.92 |
41.52 |
0.60 |
| 11 |
Patton, Calvin M (12)
|
WIMAC |
42.15 |
43.01 |
0.86 |
| 12 |
Dai, Gavin L (12)
|
WILAKE |
47.26 |
45.34 |
-1.92 |
| 13 |
Streitmatter, Samuel S (12)
|
WIMFSC |
48.42 |
45.66 |
-2.76 |
| 14 |
Sagan, Carter A (12)
|
WISWAT |
43.81 |
45.96 |
2.15 |
| 15 |
Nandakumar, Arnav (12)
|
WIEBSC |
47.89 |
45.98 |
-1.91 |
| 16 |
Bhootra, Adit (12)
|
WIMFSC |
47.47 |
46.32 |
-1.15 |
| 17 |
Buznea, Mihai (12)
|
WILAKE |
45.07 |
47.05 |
1.98 |
| 18 |
Raja, Vinod K (12)
|
WIEBSC |
47.31 |
47.28 |
-0.03 |
| 19 |
Douglas, Sam P (12)
|
WIMFSC |
49.56 |
52.17 |
2.61 |
|
Schaefer, Matthew W (12)
|
WIEBSC |
42.76 |
DQ |
- |
|
Desai, Sadev R (12)
|
WISSTY |
48.23 |
NS |
- |
|
Mishra, Avam N (12)
|
WISSTY |
|
NS |
- |