| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hauke, Emily R (16)
|
WISSTY |
3:01.03 |
3:04.37 |
3.34 |
| 2 |
Horvath, Lily C (17)
|
WIEBSC |
3:12.97 |
3:04.76 |
-8.21 |
| 3 |
Zablocki, Izzi B (15)
|
WIMWWM |
|
3:06.14 |
- |
| 4 |
Johnson, Jane E (12)
|
WIEBSC |
3:07.79 |
3:06.49 |
-1.30 |
| 5 |
Nelson, Natalie J (17)
|
WISHOR |
3:12.57 |
3:09.39 |
-3.18 |
| 6 |
McNally, Eva M (17)
|
WISHOR |
3:02.81 |
3:10.90 |
8.09 |
| 7 |
Regenfuss, Abigail L (16)
|
WIRA |
3:01.17 |
3:11.84 |
10.67 |
| 8 |
Mei, Jenny H (12)
|
WIEBSC |
3:15.62 |
3:13.55 |
-2.07 |
| 9 |
Streblow, Emma M (15)
|
WIOZ |
2:43.59 |
3:13.90 |
30.31 |
| 10 |
Dickman, Reese L (12)
|
WIWASP |
3:30.80 |
3:14.31 |
-16.49 |
| 11 |
Jester, Sara E (16)
|
WIEBSC |
3:20.11 |
3:15.29 |
-4.82 |
| 12 |
Hayward, Amelia E (17)
|
WIEBSC |
3:14.71 |
3:19.94 |
5.23 |
| 13 |
Graul, Abigail S (12)
|
WIHSSB |
3:18.48 |
3:20.04 |
1.56 |
| 14 |
Hong, Jennifer (16)
|
WIEBSC |
3:13.10 |
3:20.61 |
7.51 |
| 15 |
May, Sarah L (15)
|
WIEBSC |
3:27.56 |
3:21.19 |
-6.37 |
| 16 |
Heine, Lauryn K (12)
|
WIRA |
3:36.57 |
3:22.29 |
-14.28 |
| 17 |
Gequillana, Emily M (15)
|
WIRA |
3:36.42 |
3:34.22 |
-2.20 |
| 18 |
Morin, Madalyn G (15)
|
WIRA |
3:33.88 |
3:35.80 |
1.92 |
|
Gresnick, Felicity S (17)
|
WISHOR |
3:13.96 |
NS |
- |
|
Berger, Ainsley M (12)
|
WIEBSC |
4:18.04 |
NS |
- |