| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Guerndt, Raydan K (12)
|
WIHSSB |
46.37 |
43.73 |
-2.64 |
| 2 |
Patterson, Owen R (12)
|
WIEBSC |
48.32 |
45.39 |
-2.93 |
| 3 |
Yim, David M (11)
|
WIEBSC |
47.76 |
45.53 |
-2.23 |
| 4 |
Chen, Bruce Lyuheng L (12)
|
WISHOR |
46.59 |
45.90 |
-0.69 |
| 5 |
Prescott, Evan S (12)
|
WIEBSC |
47.62 |
46.31 |
-1.31 |
| 6 |
Li, Daniel Y (12)
|
WISSTY |
46.40 |
46.55 |
0.15 |
| 7 |
Mielnicki, Daniel C (12)
|
WISHOR |
49.06 |
49.96 |
0.90 |
| 8 |
Raymond, Jake E (12)
|
WISHOR |
50.28 |
50.70 |
0.42 |
| 9 |
Stickler, Colin E (12)
|
WIOZ |
1:01.99 |
52.32 |
-9.67 |
| 10 |
Kim, Ethan P (12)
|
WISHOR |
55.97 |
53.31 |
-2.66 |
| 11 |
Hitchcock, Everhett R (11)
|
WISSTY |
1:01.56 |
55.00 |
-6.56 |
| 12 |
Bonis, Jonathon R (12)
|
WIEBSC |
54.97 |
56.92 |
1.95 |
| 13 |
Llamazares, Mateo (12)
|
WIEBSC |
54.32 |
59.12 |
4.80 |
| 14 |
Hupfer, Jacob R (11)
|
WIRA |
1:03.06 |
1:00.82 |
-2.24 |
|
Witt, Nathan E (11)
|
WIRA |
1:00.03 |
DQ |
- |