| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Streckenbach, Kali A (8)
|
WIGBY |
47.94 |
45.09 |
-2.85 |
| 2 |
Paradis, Harper E (8)
|
WIGCY |
46.86 |
46.37 |
-0.49 |
| 3 |
Matuszewski, Kailey P (8)
|
DCNLY |
47.99 |
46.64 |
-1.35 |
| 4 |
Sund, Daylia S (8)
|
WIDCY |
43.24 |
47.01 |
3.77 |
| 5 |
Toppe, Lydia M (8)
|
WISSTY |
48.83 |
47.14 |
-1.69 |
| 6 |
Schulte, Grace (8)
|
WIMYST |
47.72 |
47.18 |
-0.54 |
| 7 |
Michalski, Amelia O (8)
|
WIDCY |
47.29 |
48.08 |
0.79 |
| 8 |
Branville, Callie (8)
|
WICVY |
50.30 |
48.13 |
-2.17 |
| 9 |
Brostowitz, Elizabeth A (7)
|
WISSTY |
49.44 |
48.45 |
-0.99 |
| 10 |
Goes, Kennedy (8)
|
WISCY |
50.37 |
48.48 |
-1.89 |
| 11 |
Fagundes, Isla V (8)
|
WIDCY |
50.56 |
48.69 |
-1.87 |
| 12 |
Chapman, Avery J (8)
|
WILAXY |
47.59 |
49.67 |
2.08 |
| 13 |
Verhelst, Maya K (8)
|
WIYDC |
50.40 |
51.15 |
0.75 |
| 14 |
Leon, Erin H (8)
|
WIWYNS |
50.16 |
51.29 |
1.13 |
| 15 |
Arnett, Lauryn R (8)
|
WIGCY |
50.80 |
51.39 |
0.59 |
| 16 |
Kort, Lydia (8)
|
WISPDY |
50.94 |
51.56 |
0.62 |
| 17 |
Knowles, Olivia (8)
|
WISCY |
49.09 |
51.90 |
2.81 |
| 18 |
Olson, Amelia E (7)
|
WIFCY |
51.13 |
52.59 |
1.46 |
| 19 |
Morrison, Kaia L (8)
|
WIGCY |
57.95 |
55.07 |
-2.88 |
| 20 |
Pella, Rebecca J (8)
|
WISSTY |
|
58.23 |
- |
| 21 |
Barber, Jenna R (8)
|
WISPDY |
1:04.54 |
58.37 |
-6.17 |
| 22 |
Thompson, Quinn I (6)
|
WILAXY |
1:07.87 |
1:03.52 |
-4.35 |
|
Goldbach, Emma R (8)
|
WIECY |
56.24 |
DQ |
- |