| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hodge, Ashley (15)
|
BORC |
26.70 |
25.90 |
-0.80 |
| 2 |
Crye, Joleigh C (15)
|
NIMERC |
26.72 |
26.49 |
-0.23 |
| 3 |
Levy, Lauren C (14)
|
NITTSC |
26.62 |
27.09 |
0.47 |
| 4 |
Van Vreede, Olivia L (15)
|
NIVICT |
28.39 |
27.62 |
-0.77 |
| 5 |
Webster, Laryssa G (15)
|
NIBRAD |
27.24 |
27.97 |
0.73 |
| 6 |
Adamczyk, Gabrielle J (13)
|
NICLSC |
27.00 |
28.58 |
1.58 |
| 7 |
Moini, Julia S (16)
|
NIBRAD |
27.87 |
28.90 |
1.03 |
| 7 |
Wiegand, Bailey M (16)
|
NITTSC |
29.11 |
28.90 |
-0.21 |
| 9 |
Costa, Maria J (13)
|
AMPWRS |
29.95 |
29.60 |
-0.35 |
| 10 |
Girts, Gracie M (16)
|
AMPWRS |
29.65 |
29.78 |
0.13 |
| 11 |
DeVore, Uriahe L (18)
|
AMPWRS |
29.96 |
29.95 |
-0.01 |
| 12 |
Yoviene, Chloe E (17)
|
NITWST |
32.27 |
30.39 |
-1.88 |
| 13 |
Natemeier, Ellie T (13)
|
AMPWRS |
30.99 |
30.41 |
-0.58 |
| 14 |
Moore, Paige E (15)
|
BORC |
40.00 |
30.59 |
-9.41 |
| 15 |
Rhoades, Isabella R (13)
|
NIUN-B |
29.66 |
30.68 |
1.02 |
| 16 |
Webster, Emma E (18)
|
NIBRAD |
30.65 |
30.87 |
0.22 |
| 17 |
Krumpek, Ella E (13)
|
NITWST |
32.30 |
32.93 |
0.63 |
| 18 |
Currier, Elizabeth D (18)
|
AMPWRS |
|
33.08 |
- |
| 19 |
Kaiser, Grace E (14)
|
NIAMTS |
36.91 |
33.37 |
-3.54 |
| 20 |
DeMeo, Isabella B (17)
|
NISTAR |
37.44 |
33.90 |
-3.54 |
| 21 |
Seelig, Jenna N (13)
|
NICLSC |
35.35 |
34.54 |
-0.81 |
| 22 |
Fernando, Kayla A (13)
|
NISHAQ |
34.38 |
34.68 |
0.30 |
| 23 |
Harrison, Ella C (13)
|
NICLSC |
36.58 |
36.07 |
-0.51 |
| 24 |
Tufan, Sadie R (15)
|
NITWST |
|
37.25 |
- |
| 25 |
Megan, Hannah E (13)
|
NIBAAC |
|
40.78 |
- |
|
Hagel, Roisin M (17)
|
NISTAR |
31.39 |
NS |
- |
|
Cartenuto, Katherine J (14)
|
NICLSC |
30.28 |
NS |
- |
|
Creamer, Laurel K (13)
|
NIVELO |
|
NS |
- |
|
Gwitt, Gretchen (15)
|
NIAMTS |
29.43 |
NS |
- |