| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Duszynski, Andrew M (24)
|
NISTAR |
22.03 |
22.81 |
0.78 |
| 2 |
Wamsley, William H (15)
|
NIVELO |
23.49 |
23.76 |
0.27 |
| 3 |
Bocci, David J (15)
|
LELESD |
24.20 |
24.17 |
-0.03 |
| 4 |
Clark, Jacob G (15)
|
LELESD |
24.48 |
24.30 |
-0.18 |
| 5 |
Matteson, Carter J (16)
|
NISTAR |
25.03 |
25.04 |
0.01 |
| 6 |
Staples, Graeme A (15)
|
NISTAR |
24.76 |
25.14 |
0.38 |
| 7 |
Strittmatter, Blaise A (15)
|
NISTAR |
25.80 |
25.91 |
0.11 |
| 8 |
Burkett, Robert O (15)
|
NISTAR |
25.94 |
26.20 |
0.26 |
| 9 |
Nienhaus, Jack (16)
|
NISHAQ |
26.76 |
26.69 |
-0.07 |
| 10 |
Eisenmann, Zachary M (15)
|
NISTAR |
27.81 |
27.04 |
-0.77 |
| 11 |
Mitchell, Jackson A (15)
|
NISTAR |
28.04 |
29.34 |
1.30 |
| 12 |
Switalski, Nicholas M (16)
|
NITTSC |
28.23 |
29.66 |
1.43 |
|
Pierce, Talyn E (16)
|
NIVICT |
23.62 |
NS |
- |