| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wamsley, Sarah E (10)
|
NIVELO |
32.53 |
32.46 |
-0.07 |
| 2 |
Anderson, Madden (10)
|
NIVELO |
33.48 |
33.68 |
0.20 |
| 3 |
Reif, Rydell (10)
|
NISHAQ |
33.29 |
33.74 |
0.45 |
| 4 |
Reukauf, Anna R (10)
|
NISTAR |
36.92 |
36.09 |
-0.83 |
| 5 |
Strittmatter, Amelia C (10)
|
NISTAR |
34.90 |
36.24 |
1.34 |
| 6 |
Marchese, Cara E (10)
|
NIBAAC |
37.56 |
37.99 |
0.43 |
| 7 |
Umansky, Isabella C (10)
|
NIBAAC |
39.44 |
38.45 |
-0.99 |
| 8 |
Wands, Lucille M (10)
|
NIVELO |
40.25 |
41.07 |
0.82 |
| 9 |
Crozier, Sydney (10)
|
NIVELO |
42.32 |
42.58 |
0.26 |
| 10 |
Tothero, Madeleine S (10)
|
NISTAR |
45.78 |
42.73 |
-3.05 |
| 11 |
Barrera-Hagner, Ananya S (10)
|
NIEAST |
38.96 |
43.73 |
4.77 |
| 12 |
Winfield, Susannah K (10)
|
NIEAST |
39.17 |
44.02 |
4.85 |
| 13 |
Horn, Erin E (10)
|
NIEAST |
44.48 |
45.09 |
0.61 |
| 14 |
Arcadi, Kayla A (10)
|
NIVELO |
43.01 |
47.85 |
4.84 |
| 15 |
Kimball, Meredith S (10)
|
NISTAR |
|
54.04 |
- |
|
Morey, Celia R (10)
|
NIBAAC |
35.91 |
NS |
- |
|
Balcom, Lucy W (10)
|
NIBAAC |
44.40 |
NS |
- |