| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keller, Mira (10)
|
NIVELO |
30.97 |
30.21 |
-0.76 |
| 2 |
Reilly, Fiona K (10)
|
NISTAR |
33.23 |
31.93 |
-1.30 |
| 3 |
Pike, Olivia R (10)
|
NISTAR |
34.50 |
33.48 |
-1.02 |
| 4 |
Wands, Amelia G (10)
|
NIVELO |
40.51 |
34.43 |
-6.08 |
| 5 |
Chung, Emily (10)
|
NIBAAC |
34.03 |
34.47 |
0.44 |
| 6 |
Kessel, Grace E (10)
|
NISHAQ |
37.80 |
38.04 |
0.24 |
| 7 |
Kreinheder, Sophie A (10)
|
NISTAR |
42.82 |
39.12 |
-3.70 |
| 8 |
Vogt, Grace A (10)
|
NISTAR |
47.86 |
39.34 |
-8.52 |
| 9 |
Moldenhauer, Paige A (10)
|
NIEAST |
40.92 |
39.67 |
-1.25 |
| 10 |
Vartanova, Elizaveta (10)
|
NIUN |
40.00 |
40.01 |
0.01 |
| 11 |
Wilson, Aria C (10)
|
NIUN |
45.31 |
40.55 |
-4.76 |
| 12 |
DelRegno, Isabella M (10)
|
NIVELO |
47.30 |
41.31 |
-5.99 |
| 13 |
Gentile, Olivia (10)
|
NIVELO |
46.82 |
41.34 |
-5.48 |
| 14 |
Peck, Violet E (10)
|
NIEAST |
40.86 |
41.91 |
1.05 |
| 15 |
Cotter, Keara L (10)
|
NISTAR |
48.25 |
46.29 |
-1.96 |
| 16 |
Brassard, Addison G (10)
|
NISTAR |
43.75 |
47.67 |
3.92 |
| 17 |
Hagel, Fiona K (10)
|
NISTAR |
50.62 |
48.36 |
-2.26 |
| 18 |
Goergen, Vanessa C (10)
|
NIEAST |
46.68 |
49.06 |
2.38 |
| 19 |
Rivera, Grace M (10)
|
NISTAR |
|
51.87 |
- |
| 20 |
Renzoni, Olivia R (10)
|
NISTAR |
|
53.41 |
- |
|
Chen, Jiayi (10)
|
NIBAAC |
49.60 |
NS |
- |
|
Bandemer, Hannah T (10)
|
NIBAAC |
44.70 |
NS |
- |