| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carter, Owen T (12)
|
NIFAST |
27.65 |
27.43 |
-0.22 |
| 2 |
LaJeunesse, Peter F (12)
|
NIVELO |
30.26 |
30.00 |
-0.26 |
| 3 |
Coen, Liam C (12)
|
NISTAR |
33.55 |
31.43 |
-2.12 |
| 4 |
Coen, Jack D (12)
|
NISTAR |
33.12 |
32.72 |
-0.40 |
| 5 |
Remick, Spencer S (12)
|
NIBAAC |
38.13 |
34.73 |
-3.40 |
| 6 |
Kobielski, Colin (12)
|
NIVELO |
38.64 |
35.54 |
-3.10 |
| 7 |
Fan, Justin F (12)
|
NICLSC |
36.83 |
35.91 |
-0.92 |
| 8 |
Kappel, Samuel A (12)
|
NIVELO |
|
36.71 |
- |
| 9 |
Burke, Ryan (12)
|
NIVELO |
46.37 |
36.86 |
-9.51 |
| 10 |
Crowther, Eli T (12)
|
NIVELO |
39.36 |
37.03 |
-2.33 |
| 11 |
Meyer, Noah (12)
|
NIVELO |
38.36 |
37.64 |
-0.72 |
| 12 |
Littrell, Alexander W (12)
|
NISTAR |
43.48 |
37.94 |
-5.54 |
| 13 |
Xie, Ethan (12)
|
NIVELO |
|
40.53 |
- |
| 14 |
Seplaki, Luke C (12)
|
NIVELO |
|
41.75 |
- |
| 15 |
Massimi-Balus, Minay E (12)
|
NISTAR |
|
57.93 |
- |
|
Amer, Siraj F (12)
|
NIVELO |
31.41 |
NS |
- |
|
Borden, Jonas O (12)
|
NIBAAC |
29.16 |
NS |
- |