| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carter, Owen T (12)
|
NIFAST |
27.43 |
27.66 |
0.23 |
| 2 |
Lemmo, Nicholas D (12)
|
NIVELO |
29.63 |
29.37 |
-0.26 |
| 3 |
LaJeunesse, Peter F (12)
|
NIVELO |
30.00 |
29.46 |
-0.54 |
| 4 |
Coen, Jack D (12)
|
NISTAR |
32.13 |
31.49 |
-0.64 |
| 5 |
Coen, Liam C (12)
|
NISTAR |
30.98 |
31.84 |
0.86 |
| 6 |
Amer, Siraj F (12)
|
NIVELO |
31.41 |
32.48 |
1.07 |
| 7 |
Remick, Spencer S (12)
|
NIBAAC |
33.52 |
33.22 |
-0.30 |
| 8 |
Kobielski, Colin (12)
|
NIVELO |
34.02 |
33.41 |
-0.61 |
| 9 |
Fan, Justin F (12)
|
NICLSC |
35.91 |
33.52 |
-2.39 |
| 10 |
DiGiacomo, Dominic J (12)
|
NICLSC |
31.85 |
33.56 |
1.71 |
| 11 |
Swan, Ethan M (12)
|
NISTAR |
36.01 |
35.26 |
-0.75 |
| 12 |
Crowther, Eli T (12)
|
NIVELO |
37.03 |
35.78 |
-1.25 |
| 13 |
Meyer, Noah (12)
|
NIVELO |
36.37 |
35.81 |
-0.56 |
| 14 |
Burke, Ryan (12)
|
NIVELO |
36.86 |
35.92 |
-0.94 |
| 15 |
Littrell, Alexander W (12)
|
NISTAR |
35.09 |
36.88 |
1.79 |
| 16 |
Woodford, Derek (12)
|
NISHAQ |
36.93 |
37.00 |
0.07 |
| 17 |
Slaughter, Timothy A (12)
|
NIVELO |
37.30 |
37.08 |
-0.22 |