| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Strittmatter, Amelia C (10)
|
NISTAR |
42.54 |
40.55 |
-1.99 |
| 2 |
Philbrick, Mylie E (10)
|
NIVELO |
42.02 |
41.09 |
-0.93 |
| 3 |
Barillari, Emma (10)
|
NIVELO |
44.47 |
42.37 |
-2.10 |
| 4 |
Zinno, Gianna M (10)
|
NICLSC |
46.14 |
42.83 |
-3.31 |
| 5 |
Gachette, Marisa B (10)
|
NIVELO |
51.98 |
43.03 |
-8.95 |
| 6 |
Weisenburg, Brylee M (10)
|
NISTAR |
45.42 |
45.16 |
-0.26 |
| 7 |
Anderson, Madden (10)
|
NIVELO |
|
47.61 |
- |
| 8 |
Umansky, Isabella C (10)
|
NIBAAC |
54.64 |
51.14 |
-3.50 |
| 9 |
Balcom, Lucy W (10)
|
NIBAAC |
|
53.19 |
- |
| 10 |
Reukauf, Anna R (10)
|
NISTAR |
56.02 |
54.14 |
-1.88 |
| 11 |
Gawron, Samantha G (10)
|
NICLSC |
|
55.46 |
- |
| 12 |
Urbanek, Addison E (10)
|
NICLSC |
53.69 |
55.98 |
2.29 |
| 13 |
Crozier, Sydney (10)
|
NIVELO |
58.97 |
59.62 |
0.65 |
| 14 |
Barrera-Hagner, Ananya S (10)
|
NIEAST |
|
1:02.23 |
- |
| 15 |
Arcadi, Kayla A (10)
|
NIVELO |
|
1:02.61 |
- |
|
Sirianni, Annabella A (10)
|
NISTAR |
|
NS |
- |
|
Tothero, Madeleine S (10)
|
NISTAR |
58.50 |
NS |
- |
|
King, Skylar L (10)
|
NIEAST |
39.85 |
NS |
- |
|
Wamsley, Sarah E (10)
|
NIVELO |
46.66 |
NS |
- |