| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carter, Owen T (12)
|
NIFAST |
32.21 |
31.63 |
-0.58 |
| 2 |
DiGiacomo, Dominic J (12)
|
NICLSC |
35.36 |
33.88 |
-1.48 |
| 3 |
LaJeunesse, Peter F (12)
|
NIVELO |
34.60 |
35.22 |
0.62 |
| 4 |
Coen, Jack D (12)
|
NISTAR |
35.05 |
35.29 |
0.24 |
| 5 |
Amer, Siraj F (12)
|
NIVELO |
34.41 |
37.75 |
3.34 |
| 6 |
Fan, Justin F (12)
|
NICLSC |
52.38 |
40.38 |
-12.00 |
| 7 |
Littrell, Alexander W (12)
|
NISTAR |
48.97 |
43.02 |
-5.95 |
| 8 |
Meyer, Noah (12)
|
NIVELO |
43.32 |
46.26 |
2.94 |
| 9 |
Swan, Ethan M (12)
|
NISTAR |
52.03 |
49.02 |
-3.01 |
| 10 |
Crowther, Eli T (12)
|
NIVELO |
55.72 |
53.67 |
-2.05 |
| 11 |
Slaughter, Timothy A (12)
|
NIVELO |
1:01.73 |
54.13 |
-7.60 |