| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wamsley, Sarah E (9)
|
NIVELO |
44.55 |
39.12 |
-5.43 |
| 2 |
Er, Melissa (9)
|
NIUN |
46.79 |
41.14 |
-5.65 |
| 3 |
Philbrick, Mylie E (9)
|
NIVELO |
46.61 |
42.12 |
-4.49 |
| 4 |
Barillari, Emma (9)
|
NIVELO |
46.35 |
45.10 |
-1.25 |
| 5 |
King, Skylar L (9)
|
NIEAST |
46.04 |
45.27 |
-0.77 |
| 6 |
Gachette, Marisa B (9)
|
NIVELO |
45.78 |
45.91 |
0.13 |
| 7 |
Maynard, Isabella M (9)
|
NIWWSC |
46.14 |
46.09 |
-0.05 |
| 8 |
Strittmatter, Amelia C (9)
|
NISTAR |
48.56 |
46.89 |
-1.67 |
| 9 |
Reukauf, Anna R (9)
|
NISTAR |
52.55 |
47.25 |
-5.30 |
| 10 |
Peiper, Reagan S (9)
|
NIBAAC |
1:02.65 |
48.23 |
-14.42 |
| 11 |
Weisenburg, Brylee M (9)
|
NISTAR |
53.50 |
48.36 |
-5.14 |
| 12 |
Tothero, Madeleine S (9)
|
NISTAR |
59.79 |
54.24 |
-5.55 |
| 13 |
Kauffman, Faith M (9)
|
NISTAR |
59.47 |
55.45 |
-4.02 |
| 14 |
Stevens, Eleanor R (9)
|
NISTAR |
|
1:09.20 |
- |
|
Barrera-Hagner, Ananya S (9)
|
NIEAST |
1:04.39 |
DQ |
- |