| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keller, Mira (11)
|
NIVELO |
32.73 |
31.77 |
-0.96 |
| 2 |
Adamczyk, Gabrielle J (11)
|
NICLSC |
35.56 |
33.88 |
-1.68 |
| 3 |
Reilly, Fiona K (11)
|
NISTAR |
34.36 |
34.51 |
0.15 |
| 4 |
McKinney, Madison N (11)
|
NICLSC |
36.09 |
35.28 |
-0.81 |
| 5 |
Mitchell, Gwenyth E (11)
|
NIEMAC |
36.48 |
35.94 |
-0.54 |
| 6 |
Swiencicki, Nora M (11)
|
NICLSC |
42.15 |
36.44 |
-5.71 |
| 7 |
Wands, Amelia G (11)
|
NIVELO |
40.16 |
38.46 |
-1.70 |
| 8 |
Mintz, Vanessa M (11)
|
NICLSC |
40.64 |
38.86 |
-1.78 |
| 9 |
Cotter, Keara L (11)
|
NISTAR |
47.32 |
40.21 |
-7.11 |
| 10 |
Vogt, Grace A (11)
|
NISTAR |
41.60 |
40.87 |
-0.73 |
| 11 |
Moldenhauer, Paige A (11)
|
NIEAST |
46.66 |
41.70 |
-4.96 |
| 12 |
Gentile, Olivia (11)
|
NIVELO |
56.03 |
43.29 |
-12.74 |
| 13 |
Nuara, Gabrielle S (11)
|
NICLSC |
45.42 |
43.46 |
-1.96 |
| 14 |
Harrison, Ella C (11)
|
NICLSC |
54.15 |
43.53 |
-10.62 |
| 15 |
Battistoni, Grace L (11)
|
NIVELO |
45.63 |
44.23 |
-1.40 |
| 16 |
Seelig, Jenna N (11)
|
NICLSC |
47.94 |
44.54 |
-3.40 |
| 17 |
Schaefer, Grace I (11)
|
NIEAST |
57.44 |
44.66 |
-12.78 |
| 18 |
Humphries, Hayden S (11)
|
NICLSC |
|
46.81 |
- |
| 19 |
Brassard, Addison G (11)
|
NISTAR |
48.33 |
47.48 |
-0.85 |
| 20 |
Boehringer, Maya L (11)
|
NICLSC |
|
47.74 |
- |
| 21 |
Booth, Sophia A (11)
|
NISTAR |
|
50.92 |
- |
| 22 |
Rivera, Grace M (11)
|
NISTAR |
49.39 |
51.32 |
1.93 |
|
Meade, Juliana E (11)
|
NISTAR |
57.21 |
DQ |
- |