| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blake, Emily C (12)
|
NISTAR |
32.95 |
31.77 |
-1.18 |
| 2 |
Tesluk, Helen E (12)
|
NISTAR |
31.72 |
32.34 |
0.62 |
| 3 |
Matyas, Morgan Q (12)
|
NISTAR |
33.45 |
34.61 |
1.16 |
| 4 |
Martino, Adrianna L (12)
|
NIWWSC |
36.25 |
34.77 |
-1.48 |
| 5 |
Shedd, Teagan E (12)
|
NISTAR |
36.16 |
35.21 |
-0.95 |
| 6 |
Hall, Sofia F (12)
|
NIBAAC |
36.27 |
36.02 |
-0.25 |
| 7 |
Flaherty, Katie T (12)
|
NIEAST |
42.94 |
38.40 |
-4.54 |
| 8 |
Kwitek, Grace M (12)
|
NIEAST |
42.30 |
38.41 |
-3.89 |
| 9 |
LI, Gloria (12)
|
NIUN |
50.00 |
39.77 |
-10.23 |
| 10 |
Truong, Jamie L (12)
|
NIUN |
50.00 |
40.26 |
-9.74 |
| 11 |
Hotchkiss, Olivia S (12)
|
NISTAR |
47.43 |
44.69 |
-2.74 |
| 12 |
Avila-Shah, Jessi E (12)
|
NIWWSC |
53.02 |
47.30 |
-5.72 |
| 13 |
Asher, Sophia (12)
|
NIVELO |
51.64 |
48.01 |
-3.63 |
| 14 |
Naim, Rita (12)
|
NIUN |
1:02.01 |
59.91 |
-2.10 |
|
Vartanova, Sofia (12)
|
NIUN |
55.00 |
DQ |
- |
|
Canestaro, Annabelle S (12)
|
NIVELO |
|
DQ |
- |
|
Zolet, Marta E (12)
|
NIEAST |
47.35 |
NS |
- |