| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Killion, Natalie A (12)
|
NICLSC |
33.62 |
30.40 |
-3.22 |
| 2 |
Leighton, Evelyn S (12)
|
NISTAR |
30.87 |
31.29 |
0.42 |
| 3 |
Pike, Olivia R (12)
|
NISTAR |
36.39 |
33.70 |
-2.69 |
| 4 |
Tesluk, Elizabeth D (12)
|
NISTAR |
32.37 |
33.90 |
1.53 |
| 5 |
Amer, Aya F (12)
|
NIVELO |
37.62 |
34.34 |
-3.28 |
| 6 |
Vaccaro, Vanessa A (12)
|
NIBAAC |
36.43 |
35.21 |
-1.22 |
| 7 |
Domster, Madeline N (12)
|
NISTAR |
37.22 |
35.61 |
-1.61 |
| 8 |
Pirigyi, Sophia J (12)
|
NISTAR |
37.46 |
36.23 |
-1.23 |
| 9 |
Lin, Isabella E (12)
|
NIVELO |
38.19 |
36.68 |
-1.51 |
| 10 |
Hull, Emily G (12)
|
NISTAR |
36.80 |
37.54 |
0.74 |
| 11 |
Denham, Myanna M (12)
|
NISTAR |
40.93 |
38.32 |
-2.61 |
| 12 |
Fosberg, Ella G (12)
|
NISTAR |
42.74 |
39.61 |
-3.13 |
| 13 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
40.36 |
39.64 |
-0.72 |
| 14 |
Brown, Haley C (12)
|
NIVELO |
|
39.85 |
- |
| 15 |
Nadarajan, Gouripriya (12)
|
NIVELO |
|
39.93 |
- |
| 16 |
Colder, Amelia W (12)
|
NISTAR |
42.69 |
41.40 |
-1.29 |
| 17 |
Morelli, Emily G (12)
|
NICLSC |
46.56 |
42.18 |
-4.38 |
| 18 |
Delille, Beatrice F (12)
|
NIVELO |
|
45.87 |
- |
| 19 |
Tothero, Katherine M (12)
|
NISTAR |
48.45 |
47.85 |
-0.60 |
|
Tripi, Haylee E (12)
|
NIVELO |
43.30 |
DQ |
- |