| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rhoades, Isabella R (12)
|
AMPWRS |
2:42.56 |
2:40.89 |
-1.67 |
| 2 |
Ramos, Hannah M (12)
|
NIRAYS |
2:53.40 |
2:44.20 |
-9.20 |
| 3 |
McKinney, Madison N (12)
|
NICLSC |
2:53.28 |
2:46.38 |
-6.90 |
| 4 |
Er, Melissa (12)
|
NIUN-D |
2:45.00 |
2:46.42 |
1.42 |
| 5 |
Ball, Lauryn R (12)
|
NIBRAD |
2:56.49 |
2:47.99 |
-8.50 |
| 6 |
Marcyan, Chloe M (12)
|
NITTSC |
3:00.57 |
2:53.40 |
-7.17 |
| 7 |
Anderson, Madden (12)
|
NIUN-0 |
3:20.56 |
2:57.89 |
-22.67 |
| 8 |
Wamsley, Sarah E (12)
|
NIVELO |
2:57.70 |
2:58.10 |
0.40 |
| 9 |
Reilly, Bridget F (11)
|
NISTAR |
3:20.20 |
3:01.12 |
-19.08 |
| 10 |
Vogt, Grace A (12)
|
NISTAR |
3:15.50 |
3:01.97 |
-13.53 |
| 11 |
Noecker, Sophia E (12)
|
NITWST |
3:17.84 |
3:02.36 |
-15.48 |
| 12 |
Scibetta, Madeline C (12)
|
NITTSC |
3:20.49 |
3:07.98 |
-12.51 |
| 13 |
Borrero, Chloe M (11)
|
AMPWRS |
|
3:08.58 |
- |
| 14 |
Seelig, Jenna N (12)
|
NICLSC |
3:34.45 |
3:09.69 |
-24.76 |
| 15 |
Gachette, Marisa B (12)
|
NIVELO |
3:20.04 |
3:10.97 |
-9.07 |
| 16 |
Strittmatter, Amelia C (12)
|
NISTAR |
3:18.39 |
3:13.69 |
-4.70 |
| 17 |
Przybysz, Tara (11)
|
NIBAAC |
|
3:13.95 |
- |
| 18 |
Murphy, Campbell E (11)
|
NITTSC |
3:33.29 |
3:17.80 |
-15.49 |
| 19 |
Philbrick, Mylie E (12)
|
NIVELO |
3:19.06 |
3:18.01 |
-1.05 |
| 20 |
Fulco, Molly E (12)
|
NIBAAC |
3:19.24 |
3:18.99 |
-0.25 |
| 21 |
Barillari, Emma (12)
|
NIVELO |
3:27.12 |
3:20.91 |
-6.21 |
| 22 |
Umansky, Isabella C (11)
|
NIBAAC |
|
3:25.86 |
- |
| 23 |
Evans, Emma C (12)
|
NIVELO |
|
3:26.33 |
- |
| 24 |
Tothero, Madeleine S (11)
|
NISTAR |
|
3:28.54 |
- |
| 25 |
Monteiro, Martha G (12)
|
NIVELO |
4:03.39 |
3:29.51 |
-33.88 |
| 26 |
Creamer, Laurel K (12)
|
NIVELO |
|
3:37.61 |
- |
| 27 |
Arcadi, Kayla A (12)
|
NIVELO |
|
3:54.83 |
- |