| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kassirer, Natalie G (12)
|
NITWST |
3:25.10 |
3:11.63 |
-13.47 |
| 2 |
Tesluk, Elizabeth D (12)
|
NISTAR |
3:14.28 |
3:13.77 |
-0.51 |
| 3 |
Keller, Mira (12)
|
NIVELO |
3:20.42 |
3:15.80 |
-4.62 |
| 4 |
Reilly, Fiona K (12)
|
NISTAR |
3:20.71 |
3:16.23 |
-4.48 |
| 5 |
Er, Melissa (11)
|
BORC |
3:20.60 |
3:17.37 |
-3.23 |
| 6 |
Marcyan, Chloe M (11)
|
NITTSC |
3:24.24 |
3:19.47 |
-4.77 |
| 7 |
Anderson, Madden (11)
|
NIVELO |
3:37.98 |
3:23.62 |
-14.36 |
| 8 |
Wands, Amelia G (12)
|
NIVELO |
3:28.25 |
3:27.73 |
-0.52 |
| 9 |
Moldenhauer, Paige A (12)
|
NIEAST |
3:50.57 |
3:28.68 |
-21.89 |
| 10 |
Pike, Olivia R (12)
|
NISTAR |
3:37.93 |
3:29.40 |
-8.53 |
| 11 |
McKinney, Madison N (11)
|
NICLSC |
3:27.63 |
3:29.86 |
2.23 |
| 12 |
Lorenz, Brie E (11)
|
NITTSC |
|
3:32.25 |
- |
| 13 |
Laspro, Julia S (12)
|
NICLSC |
|
3:34.64 |
- |
| 14 |
Tothero, Katherine M (12)
|
NISTAR |
3:55.46 |
3:38.21 |
-17.25 |
| 15 |
Lin, Isabella E (12)
|
NIVELO |
3:49.30 |
3:40.12 |
-9.18 |
| 16 |
Gachette, Marisa B (11)
|
NIVELO |
3:42.08 |
3:40.80 |
-1.28 |
| 17 |
Gracey-Dalton, Karsyn A (11)
|
NIUN-N |
3:36.80 |
3:41.31 |
4.51 |
| 18 |
Krumpek, Ella E (11)
|
NITWST |
|
3:41.85 |
- |
| 19 |
Shah, Vidya G (12)
|
NITTSC |
3:46.52 |
3:43.49 |
-3.03 |
| 20 |
Renzoni, Olivia R (12)
|
NISTAR |
3:52.71 |
3:43.84 |
-8.87 |
| 21 |
Wamsley, Sarah E (11)
|
NIVELO |
3:50.72 |
3:44.63 |
-6.09 |
| 22 |
Pauly, Ava M (12)
|
NIFALC |
|
3:44.93 |
- |
| 23 |
Noecker, Sophia E (11)
|
NITWST |
|
3:49.47 |
- |
| 24 |
Mintz, Vanessa M (12)
|
NICLSC |
4:08.49 |
3:52.43 |
-16.06 |
| 25 |
Seelig, Jenna N (11)
|
NICLSC |
3:52.61 |
3:52.53 |
-0.08 |
| 26 |
Urbanek, Addison E (11)
|
NICLSC |
|
4:01.14 |
- |
| 27 |
Adams, Daphne J (12)
|
NIUN-B |
4:14.04 |
4:07.36 |
-6.68 |
|
Reukauf, Anna R (11)
|
NISTAR |
4:06.10 |
DQ |
- |