| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carter, Owen T (12)
|
NIFAST |
35.45 |
35.45 |
0.00 |
| 2 |
LaJeunesse, Peter F (12)
|
NIVELO |
37.81 |
35.95 |
-1.86 |
| 3 |
Coen, Liam C (12)
|
NISTAR |
39.92 |
36.23 |
-3.69 |
| 4 |
Coen, Jack D (12)
|
NISTAR |
40.33 |
38.91 |
-1.42 |
| 5 |
Fan, Justin F (12)
|
NICLSC |
44.00 |
40.02 |
-3.98 |
| 6 |
Remick, Spencer S (12)
|
NIBAAC |
41.24 |
40.08 |
-1.16 |
| 7 |
Kobielski, Colin (12)
|
NIVELO |
45.41 |
41.19 |
-4.22 |
| 8 |
Meyer, Noah (12)
|
NIVELO |
44.02 |
41.56 |
-2.46 |
| 9 |
Crowther, Eli T (12)
|
NIVELO |
49.79 |
41.60 |
-8.19 |
| 10 |
Burke, Ryan (12)
|
NIVELO |
46.47 |
42.88 |
-3.59 |
| 11 |
Xie, Ethan (12)
|
NIVELO |
|
43.57 |
- |
| 12 |
Kappel, Samuel A (12)
|
NIVELO |
|
44.98 |
- |
| 13 |
Littrell, Alexander W (12)
|
NISTAR |
|
47.28 |
- |
|
Massimi-Balus, Minay E (12)
|
NISTAR |
|
DQ |
- |
|
Seplaki, Luke C (12)
|
NIVELO |
|
DQ |
- |
|
Amer, Siraj F (12)
|
NIVELO |
37.71 |
NS |
- |
|
Borden, Jonas O (12)
|
NIBAAC |
35.97 |
NS |
- |