| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Baumer, Grace E (18)
|
NIVICT |
5:24.44 |
5:21.39 |
-3.05 |
| 2 |
Matteson, Sydney A (19)
|
NISTAR |
5:09.66 |
5:24.80 |
15.14 |
| 3 |
Hawkins, Emma (13)
|
NIVICT |
5:23.55 |
5:29.14 |
5.59 |
| 4 |
Thomas, Sydney F (18)
|
NISTAR |
5:15.64 |
5:37.93 |
22.29 |
| 5 |
Moore, Madison M (14)
|
NIVICT |
5:30.05 |
5:38.78 |
8.73 |
| 6 |
Ware, Kendall E (17)
|
NIVICT |
|
5:38.88 |
- |
| 7 |
Drummond, Riley (17)
|
NITWST |
|
5:42.77 |
- |
| 8 |
Studebaker, Gabrielle W (16)
|
NISTAR |
5:30.21 |
5:45.51 |
15.30 |
| 9 |
Pakkal, Nil (13)
|
NIUN-D |
5:45.84 |
5:46.81 |
0.97 |
| 10 |
Regelsberger, Emily C (14)
|
NIVICT |
5:54.00 |
5:47.96 |
-6.04 |
| 11 |
Conley, Sarah L (15)
|
NITWST |
5:55.34 |
5:49.97 |
-5.37 |
| 12 |
Senglaub, Katie (13)
|
NIVICT |
5:38.92 |
5:50.26 |
11.34 |
| 13 |
Brinker, Mary E (15)
|
NITWST |
5:56.82 |
5:52.08 |
-4.74 |
| 14 |
Fritsch, Natalie A (12)
|
NIUN-T |
6:00.07 |
5:54.87 |
-5.20 |
| 15 |
Spence, Lauren (15)
|
NISHAQ |
5:56.60 |
5:56.30 |
-0.30 |
| 16 |
Webster, Cara F (18)
|
NIUN |
5:47.28 |
5:58.13 |
10.85 |
| 17 |
Nagy, Samantha J (18)
|
NISTAR |
5:54.16 |
5:58.57 |
4.41 |
| 18 |
Pathammavong, Suriyathai A (12)
|
NIVICT |
|
6:00.51 |
- |
| 19 |
Riccio, Emma L (15)
|
NISTAR |
5:40.81 |
6:02.25 |
21.44 |
| 20 |
Dueringer, Halle M (17)
|
NITWST |
|
6:02.53 |
- |
| 21 |
Senglaub, Emma (13)
|
NIVICT |
5:47.56 |
6:03.63 |
16.07 |
| 22 |
Mikulec, Isabella J (13)
|
NITWST |
|
6:06.76 |
- |
| 23 |
Kanner, Amelia T (16)
|
NISTAR |
6:21.00 |
6:06.96 |
-14.04 |
| 24 |
Pasquarella, Paige E (17)
|
NITWST |
|
6:08.09 |
- |
| 25 |
MacSwan, Shelby J (17)
|
NITWST |
5:54.02 |
6:08.66 |
14.64 |
| 26 |
Winseman, Isabelle A (15)
|
NIVICT |
6:01.38 |
6:09.12 |
7.74 |
| 27 |
Keller, Mira (11)
|
NIVELO |
|
6:11.13 |
- |
| 28 |
Costich, Charlotte G (13)
|
NICLSC |
6:32.30 |
6:11.64 |
-20.66 |
| 29 |
Gartner, Sarah N (15)
|
NIGCOM |
6:12.18 |
6:15.23 |
3.05 |
| 30 |
Noecker, Elle A (11)
|
NITWST |
6:37.15 |
6:16.56 |
-20.59 |
| 31 |
Prusaczyk, Victoria (16)
|
NITWST |
|
6:19.82 |
- |
| 32 |
Van Remmen, Rita A (21)
|
NISTAR |
6:17.86 |
6:20.74 |
2.88 |
| 33 |
Kisker, Elizabeth M (16)
|
NITWST |
|
6:22.28 |
- |
| 34 |
McCune, Allison G (15)
|
NITWST |
|
6:23.30 |
- |
| 34 |
McDonnell, Amelia R (12)
|
NISTAR |
6:41.00 |
6:23.30 |
-17.70 |
| 36 |
Spacciapolli, Victoria A (12)
|
NIUN-T |
6:10.21 |
6:32.25 |
22.04 |
| 37 |
Gachette, Melhea K (14)
|
NIVELO |
7:22.21 |
6:54.12 |
-28.09 |
| 38 |
Amer, Aya F (12)
|
NIVELO |
|
6:56.16 |
- |
| 39 |
Lin, Isabella E (11)
|
NIVELO |
|
6:58.90 |
- |
| 40 |
Gentile, Juliana (13)
|
NIVELO |
|
7:00.29 |
- |
| 41 |
Pitillo, Natalie E (14)
|
NISTAR |
7:02.00 |
7:03.85 |
1.85 |
| 42 |
Polek, Sarah G (13)
|
NITTSC |
|
7:59.84 |
- |
|
Kanner, Sydney G (14)
|
NISTAR |
7:08.00 |
DQ |
- |