| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Elshamy, Aly S (12)
|
NIUN-D |
28.75 |
29.40 |
0.65 |
| 2 |
Morgan, Aidan J (11)
|
NICLSC |
29.78 |
30.02 |
0.24 |
| 3 |
Gasiewicz, Aaron C (11)
|
NICLSC |
31.42 |
30.90 |
-0.52 |
| 4 |
Wong, Nicholas (12)
|
NICLSC |
33.23 |
32.45 |
-0.78 |
| 5 |
Scalfani-Cohen, Andrew J (12)
|
NIVELO |
35.14 |
34.45 |
-0.69 |
| 6 |
Bradford, Mitchell E (11)
|
LEUN-L |
33.26 |
34.70 |
1.44 |
| 7 |
Klein, Adam C (12)
|
NIVELO |
35.53 |
35.33 |
-0.20 |
| 8 |
Wissel, Paul J (11)
|
NITTSC |
35.18 |
35.45 |
0.27 |
| 9 |
Cui, Eric (12)
|
AMMIST |
43.88 |
35.80 |
-8.08 |
| 10 |
Myers, Mitchell D (11)
|
LELESD |
37.32 |
36.32 |
-1.00 |
| 11 |
Burkett, Aaron C (12)
|
NISTAR |
37.70 |
38.37 |
0.67 |
| 12 |
Priset, Kyle J (11)
|
NICLSC |
41.65 |
39.66 |
-1.99 |
| 13 |
O'Beirne, Brendan A (11)
|
NISTAR |
1:42.24 |
43.34 |
-58.90 |
| 14 |
Schoenborn, Cole D (11)
|
NICLSC |
|
43.66 |
- |
| 15 |
Clayton, Gavin M (12)
|
NISHAQ |
|
51.35 |
- |
| 16 |
Vogt, Aidan C (12)
|
NISTAR |
53.08 |
52.06 |
-1.02 |