| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Aidan R (9)
|
NIUN-D |
2:50.23 |
2:51.07 |
0.84 |
| 2 |
Chen, Ethan (10)
|
NIUN-D |
3:03.69 |
2:59.77 |
-3.92 |
| 3 |
Anderson, Max L (10)
|
NIUN-0 |
3:06.32 |
3:02.45 |
-3.87 |
| 4 |
Parsnick, William Z (9)
|
NICLSC |
3:14.54 |
3:14.65 |
0.11 |
| 5 |
Harris, Wesley S (10)
|
NIVICT |
3:15.10 |
3:16.46 |
1.36 |
| 6 |
Battaglia, Benjamin P (10)
|
NIVICT |
3:18.64 |
3:17.01 |
-1.63 |
| 7 |
Moldenhauer, Thomas C (10)
|
NIEAST |
3:01.60 |
3:20.51 |
18.91 |
| 8 |
Lamont, Dylan J (10)
|
NIVELO |
3:29.51 |
3:24.86 |
-4.65 |
| 9 |
Neal, Ducain H (9)
|
NIWWSC |
3:28.71 |
3:27.86 |
-0.85 |
| 10 |
Choi, Aaron L (10)
|
NISYCH |
3:32.12 |
3:29.63 |
-2.49 |
| 11 |
Miller, Ian M (9)
|
NIIRON |
3:38.34 |
3:31.12 |
-7.22 |
| 12 |
Nienhaus, Matthew (10)
|
NISHAQ |
3:33.44 |
3:33.70 |
0.26 |
| 13 |
Wang, Michael (10)
|
NIVELO |
3:40.49 |
3:39.20 |
-1.29 |
| 14 |
Pettitt, Brayden M (10)
|
NIWAAC |
3:36.77 |
3:47.37 |
10.60 |
| 15 |
Reimann, Nathan S (10)
|
NISTAR |
3:45.17 |
3:47.40 |
2.23 |
| 16 |
Urbanek, Trenton J (10)
|
NICLSC |
3:53.98 |
4:08.14 |
14.16 |