| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cywinski, Anna C (10)
|
NIVICT |
33.60 |
34.11 |
0.51 |
| 2 |
Loehfelm, Haley E (10)
|
NITWST |
42.08 |
39.84 |
-2.24 |
| 3 |
Burnham, Madeline (10)
|
NIVELO |
40.08 |
40.08 |
0.00 |
| 4 |
Bogunovic, Leonora (9)
|
NIVELO |
43.08 |
43.52 |
0.44 |
| 5 |
Codjovi, Jelani A (9)
|
NIVELO |
44.22 |
44.48 |
0.26 |
| 6 |
Amer, Sarah F (10)
|
NIVELO |
42.13 |
45.03 |
2.90 |
| 7 |
Pisa, Cadence E (10)
|
NIBAAC |
45.04 |
45.23 |
0.19 |
| 8 |
Martineck, Chase M (9)
|
NIVELO |
48.41 |
47.28 |
-1.13 |
| 9 |
Alessi, Luciana M (10)
|
NIBAAC |
50.15 |
49.72 |
-0.43 |
| 10 |
Zugelder, Shannon (10)
|
NIVELO |
49.55 |
50.40 |
0.85 |
| 11 |
Jermak, Amelia E (9)
|
NIBAAC |
51.06 |
50.94 |
-0.12 |
| 12 |
Wilson, Juliet (9)
|
NITWST |
52.94 |
51.29 |
-1.65 |
| 13 |
Attuwaybi, Eman (10)
|
NIVELO |
55.01 |
53.53 |
-1.48 |
| 14 |
Loehfelm, Madelyn G (8)
|
NITWST |
52.02 |
54.52 |
2.50 |
| 15 |
Garverick, Audrey D (9)
|
NIVELO |
51.32 |
54.77 |
3.45 |
| 16 |
Yost, Sydney (10)
|
NIVELO |
|
56.85 |
- |
| 17 |
Hutton, Ryan (10)
|
NIVELO |
49.88 |
57.08 |
7.20 |
| 18 |
Washington, Ashanti M (10)
|
NISTAR |
53.41 |
1:03.81 |
10.40 |
| 19 |
Ramsey, Yasmin M (9)
|
NITTSC |
1:07.82 |
1:05.96 |
-1.86 |
|
Kappel, Molly C (9)
|
NIVELO |
|
DQ |
- |
|
Malkiewicz, Heather K (10)
|
NITTSC |
43.07 |
DQ |
- |
|
Herbert, Reilly M (9)
|
NISTAR |
53.01 |
NS |
- |