| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Sophia (13)
|
NIUN-D |
5:19.00 |
5:20.55 |
1.55 |
| 2 |
Hodge, Ashley S (14)
|
NIUN-D |
5:39.99 |
5:27.16 |
-12.83 |
| 3 |
Killion, Natalie A (14)
|
NICLSC |
5:26.80 |
5:31.27 |
4.47 |
| 4 |
Switalski, Alexandra A (16)
|
NITTSC |
5:53.47 |
5:36.77 |
-16.70 |
| 5 |
Brennan, Paje (17)
|
BROC |
6:06.37 |
5:36.99 |
-29.38 |
| 6 |
Er, Melissa (12)
|
NIUN-D |
5:45.00 |
5:37.06 |
-7.94 |
| 7 |
Marcyan, Maya G (14)
|
NITTSC |
5:46.74 |
5:38.55 |
-8.19 |
| 8 |
Conley, Sarah L (17)
|
NITWST |
5:30.30 |
5:43.37 |
13.07 |
| 9 |
Moore, Kathleen E (14)
|
NIUN-D |
5:32.47 |
5:47.67 |
15.20 |
| 10 |
Marcyan, Chloe M (12)
|
NITTSC |
5:59.18 |
5:50.83 |
-8.35 |
| 11 |
Adamczyk, Gabrielle J (13)
|
NICLSC |
|
5:52.96 |
- |
| 12 |
Filipski, Julie A (16)
|
NITWST |
5:40.22 |
5:56.24 |
16.02 |
| 13 |
Sapio, Isabel J (18)
|
NITWST |
5:52.06 |
5:57.21 |
5.15 |
| 14 |
Brinker, Mary E (17)
|
NITWST |
5:47.60 |
5:58.32 |
10.72 |
| 15 |
Tesluk, Elizabeth D (13)
|
NISTAR |
5:54.14 |
5:59.39 |
5.25 |
| 16 |
Anderson, Madden (12)
|
NIUN-0 |
6:53.14 |
6:01.11 |
-52.03 |
| 17 |
Spence, Lauren (17)
|
NISHAQ |
5:42.18 |
6:03.08 |
20.90 |
| 18 |
Holland, Elle C (18)
|
NITWST |
5:57.39 |
6:03.14 |
5.75 |
| 19 |
Ramos, Hannah M (12)
|
NIRAYS |
6:49.25 |
6:16.75 |
-32.50 |
| 20 |
Roberts, Alaina G (14)
|
NITTSC |
|
6:20.18 |
- |
| 21 |
Reilly, Bridget F (11)
|
NISTAR |
|
6:24.57 |
- |
| 22 |
Griffin, Haley A (13)
|
NITTSC |
|
6:27.77 |
- |
| 23 |
Klose, Shayna (14)
|
NIAMTS |
|
6:43.90 |
- |
| 24 |
Noecker, Sophia E (12)
|
NITWST |
7:37.45 |
6:50.16 |
-47.29 |
| 25 |
Gentile, Juliana (15)
|
NIVELO |
6:38.34 |
7:00.79 |
22.45 |
| 26 |
Scibetta, Madeline C (12)
|
NITTSC |
|
7:04.01 |
- |
| 27 |
Canestaro, Annabelle S (15)
|
NIVELO |
|
7:22.65 |
- |
| 28 |
Chelpinski, Angelica J (14)
|
NIVELO |
|
7:57.88 |
- |
|
Moore, Paige E (14)
|
NIUN-D |
5:50.00 |
DQ |
- |