| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Martin, Joseph D (21)
|
NISTAR |
29.34 |
29.43 |
0.09 |
| 2 |
Acosta, Isaiah J (16)
|
NISTAR |
30.90 |
29.96 |
-0.94 |
| 3 |
Eaton, Will S (15)
|
NICLSC |
33.76 |
30.58 |
-3.18 |
| 4 |
D'Arrigo, Nicholas P (15)
|
NISHAQ |
33.71 |
32.37 |
-1.34 |
| 5 |
Strittmatter, Maximilian K (17)
|
NISTAR |
33.60 |
33.44 |
-0.16 |
| 6 |
Wang, James T (15)
|
NIUN-D |
37.63 |
33.54 |
-4.09 |
| 7 |
Blake, Joseph C (15)
|
NISTAR |
33.15 |
33.83 |
0.68 |
| 8 |
Ye, Haomin A (13)
|
NIUN-D |
45.00 |
34.72 |
-10.28 |
| 9 |
Xiao, Haoheng (14)
|
NIUN-D |
46.00 |
35.92 |
-10.08 |
| 10 |
Xiao, Xingjian (13)
|
NIUN-D |
46.00 |
38.78 |
-7.22 |
| 11 |
Herr, William A (13)
|
NIEAST |
40.05 |
39.33 |
-0.72 |