| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Daley, Lauren S (10)
|
NITWST |
3:15.98 |
3:02.80 |
-13.18 |
| 2 |
Philbrick, Mylie E (10)
|
NIVELO |
3:08.55 |
3:03.20 |
-5.35 |
| 3 |
Zagrobelny, Erin R (9)
|
NIEAST |
3:35.47 |
3:09.01 |
-26.46 |
| 4 |
Moldenhauer, Paige A (10)
|
NIEAST |
3:17.21 |
3:18.69 |
1.48 |
| 5 |
Krumpek, Ella E (10)
|
NITWST |
3:21.63 |
3:20.38 |
-1.25 |
| 6 |
Walters, Elizabeth J (10)
|
NITWST |
3:24.16 |
3:20.60 |
-3.56 |
| 7 |
Walier, Natalie K (9)
|
NITWST |
3:24.28 |
3:20.90 |
-3.38 |
| 8 |
Pecorella, Paige A (10)
|
NIEMAC |
3:23.37 |
3:22.28 |
-1.09 |
| 9 |
Sellan, Sophia K (9)
|
NITTAC |
3:45.59 |
3:27.35 |
-18.24 |
| 10 |
Umansky, Isabella C (9)
|
NIBAAC |
3:49.47 |
3:48.46 |
-1.01 |
|
Waldon, Hayden E (10)
|
NIGVST |
3:22.17 |
DQ |
- |
|
King, Skylar L (10)
|
NIEAST |
3:15.60 |
NS |
- |
|
Wamsley, Sarah E (9)
|
NIVELO |
3:06.29 |
NS |
- |