| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stockwell, Allie (16)
|
YJET |
55.02 |
58.03 |
3.01 |
| 2 |
Bernhardt, Leah A (16)
|
NICORY |
58.38 |
58.87 |
0.49 |
| 3 |
Coombs, Rachel E (17)
|
NICORY |
57.11 |
59.77 |
2.66 |
| 4 |
Haskell, Alison M (16)
|
NICORY |
59.52 |
1:01.90 |
2.38 |
| 5 |
Osborne, Grace (16)
|
YJET |
59.68 |
1:04.80 |
5.12 |
| 6 |
Butler, Emma R (15)
|
NICORY |
1:08.24 |
1:07.20 |
-1.04 |
| 7 |
Killian, Irene (15)
|
KTYC |
1:06.91 |
1:07.32 |
0.41 |
| 8 |
Gracey-Dalton, Conner N (15)
|
NIBRAD |
|
1:10.51 |
- |
| 9 |
Miles, Zara (16)
|
YJET |
1:12.32 |
1:14.89 |
2.57 |
| 10 |
Grande, Caterina (15)
|
IHYS |
1:20.84 |
1:19.33 |
-1.51 |
| 11 |
Goodrich, Emily (16)
|
ADWAT |
1:23.94 |
1:27.67 |
3.73 |