| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Acosta, Isaiah J (16)
|
NISTAR |
2:16.48 |
2:19.36 |
2.88 |
| 2 |
Martin, Joseph D (21)
|
NISTAR |
2:10.02 |
2:20.86 |
10.84 |
| 3 |
Wang, Michael (13)
|
NIUN-D |
2:29.25 |
2:26.46 |
-2.79 |
| 4 |
Murphy, Brayden (14)
|
NIVICT |
2:26.67 |
2:28.29 |
1.62 |
| 5 |
Regelsberger, Tyler R (16)
|
NIUN-V |
2:23.92 |
2:28.40 |
4.48 |
| 6 |
Clough, Michael J (17)
|
NISTAR |
2:27.56 |
2:29.63 |
2.07 |
| 7 |
Matteson, Owen K (13)
|
NISTAR |
2:30.92 |
2:30.38 |
-0.54 |
| 8 |
Clough, Andrew J (13)
|
NISTAR |
2:26.61 |
2:33.95 |
7.34 |
| 9 |
Strittmatter, Maximilian K (17)
|
NISTAR |
2:38.87 |
2:34.23 |
-4.64 |
| 10 |
Maurer, Michael O (16)
|
NISTAR |
2:33.79 |
2:35.91 |
2.12 |
| 11 |
McEwen, Andrew S (17)
|
NIVICT |
3:18.82 |
2:36.41 |
-42.41 |
| 12 |
Gangloff, Karl F (14)
|
NIUN |
2:28.77 |
2:36.71 |
7.94 |
| 13 |
Blake, Joshua C (17)
|
NISTAR |
2:27.44 |
2:37.27 |
9.83 |
| 14 |
Matteson, Carter J (15)
|
NISTAR |
2:29.29 |
2:37.70 |
8.41 |
| 15 |
Kruglov, Maxim A (13)
|
NISTAR |
2:45.83 |
2:39.77 |
-6.06 |
| 16 |
Blake, Joseph C (15)
|
NISTAR |
2:38.50 |
2:39.88 |
1.38 |
| 17 |
Burkett, Robert O (14)
|
NISTAR |
|
2:40.16 |
- |
| 18 |
Vannelli, Mitchell T (14)
|
NIUN-C |
2:42.07 |
2:41.52 |
-0.55 |
| 19 |
D'Alessandro, Kai J (14)
|
NIVICT |
2:45.78 |
2:41.94 |
-3.84 |
| 20 |
Deren, Drew E (17)
|
NISTAR |
2:39.98 |
2:42.69 |
2.71 |
| 21 |
Wang, James T (15)
|
NIUN-D |
2:46.93 |
2:43.83 |
-3.10 |
| 22 |
Staples, Graeme A (14)
|
NISTAR |
2:44.15 |
2:44.83 |
0.68 |
| 23 |
Smith, Connor J (13)
|
NIUN-V |
2:57.06 |
2:46.56 |
-10.50 |
| 24 |
Thompson, Nolan E (13)
|
NIEAST |
3:02.31 |
2:47.59 |
-14.72 |
| 25 |
Leighton, Benjamin W (13)
|
NISTAR |
3:00.84 |
2:48.70 |
-12.14 |
| 26 |
Eisenmann, Zachary M (14)
|
NISTAR |
2:45.63 |
2:51.12 |
5.49 |
| 27 |
Kibler, Clayton M (14)
|
NISTAR |
2:51.69 |
2:51.61 |
-0.08 |
| 28 |
Kibler, Seth A (14)
|
NISTAR |
2:59.51 |
2:55.66 |
-3.85 |
| 29 |
Hamilton, Christian L (16)
|
NISTAR |
3:00.82 |
2:55.76 |
-5.06 |
| 30 |
Webster, Ezra D (13)
|
NIEAST |
2:57.84 |
2:56.82 |
-1.02 |
| 31 |
Wamsley, William H (13)
|
NIVELO |
2:47.37 |
3:03.86 |
16.49 |
| 32 |
Patru, Marius (15)
|
NIVICT |
3:18.58 |
3:05.36 |
-13.22 |
| 34 |
Xiao, Xingjian (13)
|
NIUN-D |
3:30.00 |
3:07.80 |
-22.20 |
| 35 |
Thomas, Cooper D (14)
|
NISTAR |
3:11.92 |
3:09.86 |
-2.06 |
| 36 |
Egloff, Chad D (13)
|
NIEAST |
3:42.95 |
3:11.33 |
-31.62 |
| 37 |
Everett, Burke C (14)
|
NIVELO |
|
3:22.76 |
- |
| 38 |
Brod, Parker J (13)
|
NIEAST |
|
3:33.11 |
- |