| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Er, Melissa (10)
|
NIUN-D |
2:43.11 |
2:41.32 |
-1.79 |
| 2 |
Marcyan, Chloe M (10)
|
NITTSC |
2:48.46 |
2:49.44 |
0.98 |
| 3 |
Wagner, Morgan E (9)
|
NIVICT |
3:14.66 |
2:52.59 |
-22.07 |
| 4 |
Cywinski, Anna C (9)
|
NIVICT |
|
2:59.47 |
- |
| 5 |
Treleaven, Mia V (8)
|
NICLSC |
|
3:05.00 |
- |
| 6 |
Strittmatter, Amelia C (10)
|
NISTAR |
3:24.61 |
3:28.56 |
3.95 |
| 7 |
Pfalzer, Raeci A (10)
|
NIWWSC |
|
3:41.66 |
- |
| 8 |
Scibetta, Madeline (10)
|
NITTSC |
|
3:47.33 |
- |
| 9 |
Bogunovic, Leonora (8)
|
NIVELO |
|
3:49.22 |
- |
| 10 |
DelRegno, Isabella M (10)
|
NIVELO |
|
3:52.66 |
- |
| 11 |
Attuwaybi, Hibah (7)
|
NIVELO |
|
4:13.15 |
- |
| 12 |
Tothero, Madeleine S (9)
|
NISTAR |
4:09.89 |
4:15.31 |
5.42 |
| 13 |
Burnham, Madeline (8)
|
NIVELO |
4:16.53 |
4:19.01 |
2.48 |
| 14 |
Attuwaybi, Eman (8)
|
NIVELO |
|
4:25.66 |
- |