| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reilly, Bridget F (10)
|
NISTAR |
2:52.94 |
2:58.05 |
5.11 |
| 2 |
Whitley-Grassi, Ava E (10)
|
NISTAR |
|
3:18.67 |
- |
| 3 |
Murphy, Campbell E (10)
|
NITTSC |
3:14.65 |
3:18.85 |
4.20 |
| 4 |
Ryan, Isabella A (9)
|
NIVELO |
3:44.33 |
3:26.76 |
-17.57 |
| 5 |
Burnham, Madeline (9)
|
NIVELO |
3:27.53 |
3:31.94 |
4.41 |
| 6 |
Hutton, Ryan (9)
|
NIVELO |
3:42.37 |
3:32.43 |
-9.94 |
| 7 |
Alessi, Luciana M (9)
|
NIBAAC |
|
3:32.87 |
- |
| 8 |
Tothero, Madeleine S (10)
|
NISTAR |
3:31.71 |
3:43.33 |
11.62 |
| 9 |
Wands, Lucille M (10)
|
NIVELO |
3:42.72 |
3:43.41 |
0.69 |
| 10 |
Zugelder, Shannon (10)
|
NIVELO |
|
3:46.07 |
- |
| 11 |
Bogunovic, Leonora (9)
|
NIVELO |
3:39.67 |
3:46.38 |
6.71 |
| 12 |
Umansky, Katarina R (8)
|
NIBAAC |
|
3:49.11 |
- |
| 13 |
Attuwaybi, Eman (9)
|
NIVELO |
3:59.88 |
3:51.27 |
-8.61 |
| 14 |
Pisa, Cadence E (10)
|
NIBAAC |
|
3:56.28 |
- |
| 15 |
Attuwaybi, Hibah (8)
|
NIVELO |
3:51.70 |
3:57.45 |
5.75 |
| 16 |
Washington, Ashanti M (9)
|
NISTAR |
|
4:21.87 |
- |
|
Anderson, Lennon (7)
|
NIVELO |
|
NS |
- |